Molecular Characterization of Betaine Aldehyde Dehydrogenase (BADH) Gene and Proline Estimation in Hordeum vulgare L. in Response to Abiotic Stress | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Article 1, Volume 12, Issue 2, December 2020, Pages 1-21 PDF (1021.93 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2020.100593 | ||
| Authors | ||
| H. El-Atroush1; Essam A. H. Mostafa2; Samira A. Osman2 | ||
| 1Botany department, Faculty of Science, Ain Shams University, Abassia, Cairo, Egypt | ||
| 2Department of Genetics and Cytology, National Research Center, Dokki, Giza, Egypt | ||
| Abstract | ||
| Barley possesses the highest level of salt tolerance among cereals, thus it is used as an important species to investigate mechanisms involved in salt tolerance. The present investigation was conducted to study the effect of salt stress by different NaCl concentrations (0, 9000, 12000, and 15000 ppm) on proline production and it was found that there is a positive correlation between proline production and salinity. Also study the effect of salinity on BADH-1 gene expression by Real-Time PCR,where this gene was performed and it was aligned in GenBank. The sequencing was translated into amino acid sequences and these amino acid sequences were aligned in GenBank. Finally, the secondary structure of BADH-1 enzyme was determined from the most tolerant barley (Giza 2000 and Wadi Sedr) and sensitive barley Giza 129 cultivars, then sequencing translated into amino acid sequences and these sequences were aligned in GenBank. Finally, the secondary structure of BADH-1 enzyme was determined. These isolated genes were submitted in GenBank under accession numbers KX433169, KX342849, KX342850, KX342851, KX433170, and KX433171. | ||
| Keywords | ||
| Hordeum vulgare L; abiotic stress; BADH gene sequencing; proline; molecular characterization | ||
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