Bacteriological Studies on Salmonella Isolated from Balady Chicken Meat | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 6, Volume 25, Issue 1, June 2020, Pages 105-114 PDF (517.58 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2020.103013 | ||
| Authors | ||
| Hamza Eid1; Ihab Helal2; Radwa Kouta* 3 | ||
| 1Bacteriology, Immunology and Mycology Department, Fac. Vet. Med. Suez Canal University | ||
| 2Animal Health Research Institute, Port Said branch, ARC. | ||
| 3Suez Canal University Faculty of Veterinary Medicine Bacteriology, Immunology and Mycology Department | ||
| Abstract | ||
| Two hundred freshly slaughtered of balady chicken samples were collected aseptically from markets in Port-Said city during the period from September 2016 to September 2018. Nine Salmonella isolates (4.5%) were isolated and therefore serologically identified. The isolated Salmonellae were Salmonella Typhimurium 2/9(22.2%) and SalmonellaMuenster 2/9 (22.2%) which was the most frequently identified. Other serotypes as Salmonella Enteritidis 1/9 (11.1%), Salmonella Blegdam 1/9 (11.1%), Salmonella Anatum1/9(11.1%), Salmonella Lamberhurst1/9(11.1%) and Salmonella Ayinde 1/9 (11.1%) were also identified. The antibiogram revealed highly resistant to Erythromycin and Oxytetracycline by a percentage (100%) followed by Nalidixic acid (44.4%) while it was highly sensitive to Chloramphenicol and Ciprofloxacin by a percentage (100%) followed by Gentamycin (88.9%). Polymerase Chain Reaction detected the virulence genes (stn, sop < /em>B, spvC and bcfC) genes were positive in all tested Salmonella serotypes 7/7 (100%) while (avrA) gene was positive in 6/7 (85.7%) of tested Salmonella serotypes. | ||
| Keywords | ||
| Salmonella; chicken; PCR; Antibiogram sensitivity; Port; Said | ||
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