Ahmed, W., Abd El-Tawab, A., Elhofy, F., Hotzel, H., Sobhy, M. (2019). Phenotypic and genotypic characterization of Campylobacter isolated from poultry. test, 37(2), 33-36. doi: 10.21608/bvmj.2020.22927.1156
wedad Ahmed; Ashraf Awad Abd El-Tawab; Fatma Elhofy; Helmut Hotzel; Mona Sobhy. "Phenotypic and genotypic characterization of Campylobacter isolated from poultry". test, 37, 2, 2019, 33-36. doi: 10.21608/bvmj.2020.22927.1156
Ahmed, W., Abd El-Tawab, A., Elhofy, F., Hotzel, H., Sobhy, M. (2019). 'Phenotypic and genotypic characterization of Campylobacter isolated from poultry', test, 37(2), pp. 33-36. doi: 10.21608/bvmj.2020.22927.1156
Ahmed, W., Abd El-Tawab, A., Elhofy, F., Hotzel, H., Sobhy, M. Phenotypic and genotypic characterization of Campylobacter isolated from poultry. test, 2019; 37(2): 33-36. doi: 10.21608/bvmj.2020.22927.1156

Phenotypic and genotypic characterization of Campylobacter isolated from poultry

Benha Veterinary Medical Journal
Article 6, Volume 37, Issue 2, December 2019, Pages 33-36    PDF (770.26 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2020.22927.1156
Authors
wedad Ahmed* 1; Ashraf Awad Abd El-Tawab2; Fatma Elhofy2; Helmut Hotzel3; Mona Sobhy4
1department of bacteriology, immunology and mycology,faculty of veterinary medicine ,Benha University
2Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
3Friedrich loeffler institiute ,Jena ,Germany
4Department of Reproductive Diseases, ARRI, ARC, 12556 Giza, Egypt
Abstract
Campylobacteriosis incidence and proportion of Campylobacter strains resistant to antibiotic has been increasing worldwide in the last decades. Campylobacteriosis is considered as one of the major important zoonotic gastrointestinal diseases around the world. The aim of this study is isolation and biochemical characterization of Campylobacter from poultry. Campylobaceter isolation have been done on MCCDA media, motility have been detected by phase contrast microscope , morphology have been detected by Gram stain, and confirmed by MALDI-TOF MS and molecular confirmation by using cPCR by amplification of 16SrRNA gene. A total of 102 isolate of Campylobacter were isolated from (farm and small backyards located in different sources in El-Kalyobia , El-Monofia and El-sharkia Governorate) by conventional bacteriological methods from which 40 were C.jejuni and 62 were C.coli. All isolats have been confirmed as Campylobacter by MALDI-TOF MS and detection of 16SrRNA were detected by cPCR in all isolates. PCR is a useful molecular tool for identification and confirmation of Campylobacter, its Rapid, sensitive and specific than the culture methods the only disadvantage is that is expensive.
Keywords
Campylobaceter; poultry; prevelance
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