El-Shabouri, S., Ibrahim, S. (1980). RAPID, SENSITIVE COLORIMETRIC ASSAY FOR ISOPRENALINE. test, 3(1), 125-136. doi: 10.21608/bfsa.1980.104723
Salwa R. El-Shabouri; S. A. Ibrahim. "RAPID, SENSITIVE COLORIMETRIC ASSAY FOR ISOPRENALINE". test, 3, 1, 1980, 125-136. doi: 10.21608/bfsa.1980.104723
El-Shabouri, S., Ibrahim, S. (1980). 'RAPID, SENSITIVE COLORIMETRIC ASSAY FOR ISOPRENALINE', test, 3(1), pp. 125-136. doi: 10.21608/bfsa.1980.104723
El-Shabouri, S., Ibrahim, S. RAPID, SENSITIVE COLORIMETRIC ASSAY FOR ISOPRENALINE. test, 1980; 3(1): 125-136. doi: 10.21608/bfsa.1980.104723

RAPID, SENSITIVE COLORIMETRIC ASSAY FOR ISOPRENALINE

Bulletin of Pharmaceutical Sciences Assiut University
Article 11, Volume 3, Issue 1, 1980, Pages 125-136    PDF (426.95 K)
Document Type: Original Article
DOI: 10.21608/bfsa.1980.104723
Authors
Salwa R. El-Shabouri1; S. A. Ibrahim2
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt
2Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
Abstract
A simple, rapid, selective and highly sensitive colorimetric method for the determination of isoprenaline (isoproterenol) is described. The method depends on the interaction of dimethoxydiquinone, with isoprenaline sulphate under specified conditions with the formation of a highly coloured, product which has a lmax at 5l0 nm and apparent molar absorptivity of 3.8x106. Absorbance versus concentration is linear up to 6 mcg/ml. No interference is observed in presense of common pharmaceutical adjuvants. The developed method is applicable with almost complete recovery for assaying isoprenaline in commercial, pharmaceutical dosage forms without prior separation. The proposed method is also recommended as stability indicating assay for oxidative degradation involving the catecholic  function of isoprenaline.
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