DIRECT COLORIMETRIC ANALYSIS OF STILBESTROL DIPROPIONATE | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Article 2, Volume 1, Issue 1, 1978, Pages 15-33 PDF (672.74 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.1978.104905 | ||
| Authors | ||
| Nabil M. Omar; Nawal A. El-Rabbat | ||
| Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||
| Abstract | ||
| A convenient and specific spectrophotometric determination of stilbestrol and stilbestrol dipropionate is developed by interaction with 2,3,5-triphenyltetrazolium chloride at 50°C for 45 minutes and subsequent measurement of the color formed at 485 nm. Evidence is provided to account for the significance of extended conjugation of 4,4'-stilbenediol derivatives to induce this color reaction. Ideal adherence of color absorption to Beer's law permitted accurate and precise determination of stilbestrol and stilbestrol dipropionate pure forms over a range of 2-22 mcg stilbestrol/ml. Application of the tetrazolium, reaction to the analysis of stilbestrol dipropionate oily dosage forms could be satisfactorily effected without prior hydrolytic or extraction processes. This offers a fundamental achievement for utility of the proposed procedure in automated analysis. | ||
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