Ojo, A., Adebajo, S., Fasina, K., Odejinmi, B., Ojo, O., Ooladotun, A., Ibeaha, J. (2021). Phenotypic detection of carbapenem resistant Enterobacteriaceae and characterization of Klebsiella pneumoniae carbapenemase(KPC) producing Klebsiella pneumonia. test, 2(4), 760-766. doi: 10.21608/mid.2020.36999.1040
Abidemi Esther Ojo; Seun Owolabi Adebajo; kolapo A Fasina; Bolutife Odejinmi; Olugbenga anthony Ojo; abiola Ooladotun; JOSHUA Ibeaha. "Phenotypic detection of carbapenem resistant Enterobacteriaceae and characterization of Klebsiella pneumoniae carbapenemase(KPC) producing Klebsiella pneumonia". test, 2, 4, 2021, 760-766. doi: 10.21608/mid.2020.36999.1040
Ojo, A., Adebajo, S., Fasina, K., Odejinmi, B., Ojo, O., Ooladotun, A., Ibeaha, J. (2021). 'Phenotypic detection of carbapenem resistant Enterobacteriaceae and characterization of Klebsiella pneumoniae carbapenemase(KPC) producing Klebsiella pneumonia', test, 2(4), pp. 760-766. doi: 10.21608/mid.2020.36999.1040
Ojo, A., Adebajo, S., Fasina, K., Odejinmi, B., Ojo, O., Ooladotun, A., Ibeaha, J. Phenotypic detection of carbapenem resistant Enterobacteriaceae and characterization of Klebsiella pneumoniae carbapenemase(KPC) producing Klebsiella pneumonia. test, 2021; 2(4): 760-766. doi: 10.21608/mid.2020.36999.1040

Phenotypic detection of carbapenem resistant Enterobacteriaceae and characterization of Klebsiella pneumoniae carbapenemase(KPC) producing Klebsiella pneumonia

Microbes and Infectious Diseases
Article 19, Volume 2, Issue 4, November 2021, Pages 760-766    PDF (255.48 K)
Document Type: Original Article
DOI: 10.21608/mid.2020.36999.1040
Authors
Abidemi Esther Ojo* 1; Seun Owolabi Adebajo2; kolapo A Fasina3; Bolutife Odejinmi2; Olugbenga anthony Ojo4; abiola Ooladotun2; JOSHUA Ibeaha2
1department of Microbiology, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria
2Microbiology Department, College of Biosciences, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun State, Nigeria.
3Biotechnology Division, Rubber Research Institute of Nigeria, PMB 1049, Iyanomo, Benin City, Edo State, Nigeria
4Department of Pharmacognosy, Faculty of Pharmacy, Madonna University, Port Harcourt, Rivers State, Nigeria
Abstract
Background: Carbapenem-resistant Enterobacteriaceae (CRE) pose a great threat to the public health sector as a result of its high communicability, high morbidity and mortality rates. This study investigated the occurrence of carbapenem resistant Enterobacteriaceae and the prevalence of Klebsiella pneumoniae carbapenemase (KPC) producing Klebsiella pneumoniae as a mechanism of resistance. Method: One hundred and ten Enterobacteriaceae isolates were cultured from different samples of patients in Intensive Care Units of two hospitals. Using Clinical and Laboratory Standards Institute (CLSI) Guidelines of 2017. Carbapenemase production was determined phenotypically using Brilliance CRE agar, Carbapenem inactivation method and confirmed with Modified Hodge test (MHT).  Modified Hodge test positive isolates were screened for KPC-producing Klebsiella pneumoniae using boronic acid-based inhibition test. Result: Phenotypic results revealed an occurrence of 10.9% (12/110) isolates for carbapenem resistant Enterobacteriaceae while KPC-producing Klebsiella pneumoniae prevalence was 3.6% contributing 33.3% to the burden of carbapenem production. The occurrence of carbapenem-resistant Enterobacteriaceae in this study was relatively high and KPC-Klebsiella pneumoniae had a significant contribution to the burden of CRE. Conclusion: Hence, a sinewy antibiotic stewardship is needed in this regard.
Keywords
Carbapenem resistant enterobactereaceae; Modified hodge test; Boronic acid-based inhibition test; Klebsiella pneumoniae carbapenemase
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