Khalil, A., Radwan, N., Osman, G., Abo Msalam, A., Maghrabi, O. (2016). In vitro Effect of LC90 of albendazole and Allium sativum water extract on the fine structure of Capillaria sp. (Capillaridae Nematoda).. test, 2(2), 125-135. doi: 10.21608/jbaar.2016.106916
Amal I. Khalil; Nahla A. Radwan; Gamalat Y. Osman; Alyaa M. Abo Msalam; Omaimah A. M. Maghrabi. "In vitro Effect of LC90 of albendazole and Allium sativum water extract on the fine structure of Capillaria sp. (Capillaridae Nematoda).". test, 2, 2, 2016, 125-135. doi: 10.21608/jbaar.2016.106916
Khalil, A., Radwan, N., Osman, G., Abo Msalam, A., Maghrabi, O. (2016). 'In vitro Effect of LC90 of albendazole and Allium sativum water extract on the fine structure of Capillaria sp. (Capillaridae Nematoda).', test, 2(2), pp. 125-135. doi: 10.21608/jbaar.2016.106916
Khalil, A., Radwan, N., Osman, G., Abo Msalam, A., Maghrabi, O. In vitro Effect of LC90 of albendazole and Allium sativum water extract on the fine structure of Capillaria sp. (Capillaridae Nematoda).. test, 2016; 2(2): 125-135. doi: 10.21608/jbaar.2016.106916

In vitro Effect of LC90 of albendazole and Allium sativum water extract on the fine structure of Capillaria sp. (Capillaridae Nematoda).

Journal of Bioscience and Applied Research
Article 6, Volume 2, Issue 2, February 2016, Pages 125-135    PDF (1010.28 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2016.106916
Authors
Amal I. Khalil1; Nahla A. Radwan1; Gamalat Y. Osman2; Alyaa M. Abo Msalam2; Omaimah A. M. Maghrabi3
1Zoology Department, Faculty of science, Tanta University, Tanta, Egypt
2Zoology Department, Faculty of science, Menoufia University, Menoufia, Egypt
3Biology Department, Faculty of science, Umm Al Qura University, Makkah, Saudi Arabia
Abstract
Fish parasites are of economic and health importance where they cause serious problems in commercial fish farms and it can be transferred to human and animals. Capillaria species are nematodes of serious fish diseases which may be transferred to human.The present study was conducted to detect the in vitro effect of sublethal concentration (LC90) of albendazole( ABZ ) and Allium sativum (garlic) cloves water extract on ultra structure of Capillaria sp. collected from fresh water catfishes, Bagrus docmac and B. bayad using SEM and TEM. The morality rates of the adult Capillaria sp. in vitro exposed to different concentrations of albendazole and Allium sativum water extract was recorded after 24 hours exposure time. A. sativum water extract had higher effect than albendazole where lower concentration of A. sativum extract (18 x103 ppm) gave maximum mortality rate (100%) , 24 hours post exposure. SEM studies of the cuticle of adult Capillaria sp. in vitro treated with LC90 (17.161 x103 ppm) of A. sativum water extract for 24 hours revealed that it induced severe changes in the cuticle integrity more than those treated with concentration LC90 (5.543 x 106 ppm) of albendazole. TEM studies revealed deformation of the cuticle, hypodermis, muscles and digestive system in worms treated with LC90 (17.161 x103 ppm) of Allium sativum water extract.
Keywords
Capillaria; Albendazole. In vitro; Allium sativum; SEM; TEM
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