Effect of Intra-Articular Injection of Chondrocytes Differentiated from Mesenchymal Stem Cells in Monosodium iodoacetate Induced Osteoarthritis in Male Rats | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 1, Volume 41, Issue 2, April 2021, Pages 144-156 PDF (1.11 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2020.29649.1059 | ||
| Authors | ||
| Maha M ElBatsh* 1; Rawhia Eledel2; Rasha Ibrahim Noreldin3; Asmaa Shams El Dein Mohamed4; Thoria Ahmed Omar3 | ||
| 1Clinical Pharmacology Department, Faculty of Medicine, Menoufia University, Shebin ElKom, Egypt | ||
| 2Clinical Pathology Department , Faculty of Medicine, Menoufia University, Shebin ElKom, Egypt | ||
| 3Clinical Pathology Department, Faculty of Medicine, Menoufia University, Shebin ElKom, Egypt | ||
| 4pathology departement, faculty of medicine, menoufia university | ||
| Abstract | ||
| Abstract Osteoarthritis [OA] is a chronic, prevalent, debilitating joint disease characterized by progressive cartilage degradation, subchondral bone remodeling, bone marrow lesions, meniscal damage, and synovitis. Joint diseases are very difficult to be treated with traditional line therapy. Hence the importance of developing new therapeutic methods such as stem cell therapy. Mesenchymal Stem Cells [MSCs] isolated from multiple tissues as bone marrow, adipose tissue, dental pulp, placenta and umbilical cord. They possess ability of self-renewal and multilineage differentiation which is a hoping tool for treatment of multiple diseases. The aim of this study is to assess the ability of stem cells to differentiate into chondrocytes and the ability of those chondrocytes to induce cartilage repair and suppresses cartilage degeneration in Monosodium iodoacetate [MIA] induced osteoarthritis in rats' model. In this experimental study Wharton jelly separated from umbilical cord was used to isolate MSCs then differentiated to chondrocytes and injected into MIA induced osteoarthritis in rat's model. The rats divided into three groups; control group, MIA-treated group and MIA- Chondrocytes treated group. Results: MIA- Chondrocytes treated group appeared healthy with no signs of inflammation. On microscopic examination: the articular cartilage of injected limbs showed observable restoration of surface continuity, chondrocytes density, boundaries between layers and matrix basophilia. Conclusion: Intra-articular injection of chondrocytes improves, inhibits the progression of degeneration of the affected cartilage and induces cartilage repair. | ||
| Keywords | ||
| Wharton jelly; mesenchymal stem cells; Chondrocytes; Osteoarthritis | ||
|
Statistics Article View: 454 PDF Download: 388 |
||
