UTILITY OF PCR ASSAY FOR DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN CLINICAL SAMPLES FROM PERSISTENTLY INFECTED CATTLE | ||
| Kafrelsheikh Veterinary Medical Journal | ||
| Article 36, Volume 7, Issue 1, May 2009, Pages 648-668 PDF (1.26 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/kvmj.2009.108711 | ||
| Author | ||
| G. S. Radwan* | ||
| Genetic Engineering and Biotechnology Research Institute, Minufiya University, Sadat City, Egypt | ||
| Abstract | ||
| In this study, a polymerase chain reaction (PCR) assay was used for the detection of bovine viral diarrhea virus (BVDV) persistent infections in cattle. BVDV RNA's from individual serum, milk and semen clinical specimens from persistently infected (PI) cattle were transcribed to cDNA using reverse transcriptase. Using a set of oligonucleotide primers complementary to nucleotide sequence within the conserved 5' untranslated region (UTR) of the BVDV genome, a 246 base pair target sequence from BVDV cDNA from clinical samples was successfully amplified by PCR. The sensitivity of PCR detection of BVDV nucleic acid was 10 times more than that of BVDV isolation from serum of PI animals. The results suggest that PCR amplification assay may be a useful addition in developing new rapid and sensitive tests for detection of BVDV in clinical samples from PI animals. The speed and the sensitivity of this method might be of value for perusing studies on pathogenesis and epidemiology of persistent infection of cattle with BVD virus. | ||
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| Keywords | ||
| PCR; BOVINE VIRAL DIARRHEA VIRUS; PERSISTENTLY; CATTLE | ||
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