El Sawaak, A., El Desoky, I., Abd Elgwaad,, A., Ahmed, H., Shalaby, M. (2016). DETECTION OF VIRULENCE ASSOCIATED GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM DISEASED FARM ANIMALS. test, 14(1), 297-314. doi: 10.21608/kvmj.2016.108869
Ahmed A. El Sawaak; Ibrahim El Desoky; Ahmed M Abd Elgwaad,; Hanaa A. Ahmed; Marwa I. E. Shalaby. "DETECTION OF VIRULENCE ASSOCIATED GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM DISEASED FARM ANIMALS". test, 14, 1, 2016, 297-314. doi: 10.21608/kvmj.2016.108869
El Sawaak, A., El Desoky, I., Abd Elgwaad,, A., Ahmed, H., Shalaby, M. (2016). 'DETECTION OF VIRULENCE ASSOCIATED GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM DISEASED FARM ANIMALS', test, 14(1), pp. 297-314. doi: 10.21608/kvmj.2016.108869
El Sawaak, A., El Desoky, I., Abd Elgwaad,, A., Ahmed, H., Shalaby, M. DETECTION OF VIRULENCE ASSOCIATED GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM DISEASED FARM ANIMALS. test, 2016; 14(1): 297-314. doi: 10.21608/kvmj.2016.108869

DETECTION OF VIRULENCE ASSOCIATED GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM DISEASED FARM ANIMALS

Kafrelsheikh Veterinary Medical Journal
Article 18, Volume 14, Issue 1, May 2016, Pages 297-314    PDF (794.21 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2016.108869
Authors
Ahmed A. El Sawaak* 1; Ibrahim El Desoky2; Ahmed M Abd Elgwaad,3; Hanaa A. Ahmed4; Marwa I. E. Shalaby3
1Pathology Dept. Faculty of Veterinary Medicine Kafrelsheikh University, Egypt.
2Bacteriology, Mycology and Immunology Dept. Fac. of Vet. Med. Kafrelsheikh Univ., Egypt.
3Animal health research institute, Mansoura Provincial Laboratory.
4Genome Research Unite, AHRI
Abstract
A total of 631 different samples (milk, faeces, brain andCerebrospinal fluid) were collected from 268 diseased farm animals and emergency slaughtered animals (80 sheep, 60goat, 75 cattle and 53 buffaloes) from Dakahlia Governoratein 2014 and 2015. The samples were tested for presence of L. monocytogenes by isolation and biochemical identification.
 The results revealed that L. monocytogenes was presented in diseased and emergency slaughtered sheep, goat, cattle and buffaloes in ratio of (36.5%), (28.67%), (24.48%), (17.4%) respectively. Using multiplex PCR technique, detected the presence of virulence-associated genes (inlA, inlC, inlJ, inlB, plcB, hylA, jap and plcA).
Keywords
L. monocytogenes; virulence-associated genes
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