Ameliorative effect of Echinacea purpurea and curcumin on dexamethasone-induced immunosuppressive rabbits | ||
| Kafrelsheikh Veterinary Medical Journal | ||
| Article 3, Volume 18, Issue 1, August 2020, Pages 10-16 PDF (373.67 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/kvmj.2020.109085 | ||
| Authors | ||
| Azza A. Ibrahim* 1; Rasha M Saleh2; Azza E. Hassan1; Magdy S. Amer3 | ||
| 1Biochemistry Department, Animal Health Research Institute, Mansoura, Egypt | ||
| 2Physiology Department, Faculty of Veterinary Medicine, Mansoura University, Egypt | ||
| 3Pharmacology Department, Faculty of Veterinary Medicine, Mansoura University | ||
| Abstract | ||
| Objectives: This study was delineated to investigate the ameliorative effect of Echinacea purpurea and curcumin against oxidative stress and immunosuppression induced by dexamethasone in rabbits. Methods: A total of 20 healthy rabbits were used in this study and were randomly classified into 4 groups of 5 each. The 1st group (non-treated) was served as a control, the 2nd group intramuscularly medicated with dexamethasone (2 mg/kg) 3 times at 6 hours interval, the 3rd group medicated with dexamethasone at the same dose then received E. purpurea (130 mg/kg) orally day after day for 21 days, and the 4th group medicated with dexamethasone at the same dose then received curcumin (400 mg/kg) orally every day for 21 days. Blood and serum samples were collected after 1, 7, and 14 days post-dosing. Results: Dexamethasone medicated group showed a significant decrease in WBCS, lymphocytes, neutrophils, and monocytes at the 1st, 7th, and 14th day, but E. purpurea and curcumin medicated groups showed significant improvement in total and differential leucocytic count. Also, both plants showed a significant increase in lysozymes and nitric oxide against the suppressive effect of dexamethasone. Here, the dexamethasone group showed a significant decrease in serum catalase (CAT) and significant elevation in serum malondialdehyde (MDA) at 1st and 7th-day post-treatment. E. purpurea and curcumin groups showed a significant increase in serum CAT and significant depletion in MDA levels. Injection of dexamethasone significantly decreased the serum levels of total protein, albumin, and globulin as compared to the control group. These parameters were significantly increased after treatment with E. purpurea and curcumin. Treatment with E. purpurea or curcumin ameliorated tissue damages of liver, kidney, and spleen induced by dexamethasone. Conclusion: Treatment with Echinacea purpurea or curcumin could ameliorate oxidative stress and immunosuppression induced by dexamethasone in rabbits. | ||
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| Keywords | ||
| Echinacea purpurea; curcumin; oxidative stress; immunomodulatory; rabbit | ||
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