Kasem, S., Albagory, G., Abdelkader, A., Fukushi, H., Alkhalefa, N., Nayel, M., Kamal, T., Dawod, A. (2015). IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1. test, 13(1), 35-55. doi: 10.21608/kvmj.2015.109535
Samy Kasem; Gabr Albagory; Ali Abdelkader; Hideto Fukushi; Noura Alkhalefa; Mohamed Nayel; Tarek Kamal; Al-Said Dawod. "IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1". test, 13, 1, 2015, 35-55. doi: 10.21608/kvmj.2015.109535
Kasem, S., Albagory, G., Abdelkader, A., Fukushi, H., Alkhalefa, N., Nayel, M., Kamal, T., Dawod, A. (2015). 'IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1', test, 13(1), pp. 35-55. doi: 10.21608/kvmj.2015.109535
Kasem, S., Albagory, G., Abdelkader, A., Fukushi, H., Alkhalefa, N., Nayel, M., Kamal, T., Dawod, A. IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1. test, 2015; 13(1): 35-55. doi: 10.21608/kvmj.2015.109535

IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1

Kafrelsheikh Veterinary Medical Journal
Article 3, Volume 13, Issue 1, April 2015, Pages 35-55    PDF (910 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2015.109535
Authors
Samy Kasem* 1; Gabr Albagory2; Ali Abdelkader3; Hideto Fukushi4; Noura Alkhalefa1; Mohamed Nayel5; Tarek Kamal6; Al-Said Dawod7
1Department of Virology, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt
2Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt
3Department of Anatomy, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt
4Department of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
5Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City University, Sadat City, Egypt
6Department of Biochemistry, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt
7Animal research institute, Kafrelsheikh branch, El Geish Street, Kafrelsheikh, Egyp
Abstract
UL45 was one of the tegument proteins that have been identified in both EHV-1 and EHV-4, it was recorded that ORF 15 EHV-1was homologues to UL45 (HSV). Until now, the function of UL45 protein in cell culture and animal models is poorly understood. To investigate the function of the UL45 protein, we expressed the UL45 gene in prokaryotic expression vector pGEX-6P-1 to express UL45 protein and used the resulting protein to raise guinea pig antiserum. In western blotting experiments, the antiserum directed against the N-terminal of EHV-1 UL45 protein specifically recognized a protein with molecular weight ranging from 30 to 60 kDa in the infected cells lysates. The immunofluorescence assay also showed that EHV-1 ORF15 antiserum was able to probe the UL45 protein which is localized mainly in the cytoplasm associating with the membrane of the cells infected with EHV-1 Ab4p.
Keywords
Equine herpes virus -1 (EHV-1); UL45 protein; Protein expression; Polyclonal antibody; Immunofluorescence assay
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