MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT | ||
| Scientific Journal of Flowers and Ornamental Plants | ||
| Article 3, Volume 7, Issue 3, September 2020, Pages 239-246 PDF (324.4 K) | ||
| Document Type: Original Research Article | ||
| DOI: 10.21608/sjfop.2020.110364 | ||
| Authors | ||
| Hany Mohamed samy Hassan* 1; M. Moubarak2 | ||
| 1Plant production department, Faculty of Environment Agricultural Sciences, Arish University | ||
| 2Department of Horticulture, Fac. Agric., Damanhour Univ., Damanhour City, Egypt | ||
| Abstract | ||
| The present study aims to determine the best type and concentration of growth regulators and find cheap and/or readily-available alternative substitutes of agar for tissue culture media preparation. The results clear that MS medium supplemented with 1 mg l-1 BA significantly outperformed other tested cytokinins concerning yucca shoot multiplication and growth parameters (number of shoots/explant, number of leaves/shoot, and shoot length (cm)) which recorded 4.75, 14.00 and 6.50 cm, respectively. Moreover, the combination between 2 g l-1 agar and 6 g l-1 guar as gelling agents produced the maximum shoot growth values. During rooting stage, data clear that 1.0 mg l-1 NAA increased number of leaves/shoot, number of roots/shoot and root length with mean values of 20.50, 3.75 and 5.22 cm, respectively. Finally, rooted plantlets were successfully acclimatized with maximum survivability percentage (100%) in soil mixture of sand and peat moss (1:1. v/v). | ||
| Keywords | ||
| Yucca; BA; gelling agent; guar seeds powder; NAA | ||
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