Nasr, ,., Fawzy, R., Marian., G., Khalifa, E., Abbas, A. (2020). Sero-diagnosis of Bovine tuberculosis using a rapid lateral flow test and ELISA in dairy cattle in Egypt. test, 3(7), 75-100. doi: 10.21608/asajs.2020.113636
, E Nasr; R. Fawzy; G. S Marian.; Eman Khalifa; A.M. Abbas. "Sero-diagnosis of Bovine tuberculosis using a rapid lateral flow test and ELISA in dairy cattle in Egypt". test, 3, 7, 2020, 75-100. doi: 10.21608/asajs.2020.113636
Nasr, ,., Fawzy, R., Marian., G., Khalifa, E., Abbas, A. (2020). 'Sero-diagnosis of Bovine tuberculosis using a rapid lateral flow test and ELISA in dairy cattle in Egypt', test, 3(7), pp. 75-100. doi: 10.21608/asajs.2020.113636
Nasr, ,., Fawzy, R., Marian., G., Khalifa, E., Abbas, A. Sero-diagnosis of Bovine tuberculosis using a rapid lateral flow test and ELISA in dairy cattle in Egypt. test, 2020; 3(7): 75-100. doi: 10.21608/asajs.2020.113636

Sero-diagnosis of Bovine tuberculosis using a rapid lateral flow test and ELISA in dairy cattle in Egypt

المجلة العربية للعلوم الزراعية
Article 4, Volume 3, Issue 7, July 2020, Pages 75-100    PDF (1.13 M)
Document Type: المقالة الأصلية
DOI: 10.21608/asajs.2020.113636
Authors
, E Nasr1; R. Fawzy2; G. S Marian.3; Eman Khalifa4; A.M. Abbas5
1Department of bacterial diagnostic product (TB), Veterinary Serum and Vaccine Research Institute, Cairo, Egypt.
2.Professor of Microbiology Department, Faculty of Veterinary Medicine Beni-Suif University.
3Microbiology Department, Faculty of Veterinary Medicine Beni-Suif University.
4Department of Microbiology, Faculty of Veterinary Medicine, Matrouh University
5Head of Genetic Engineering Research Department (GERD) Veterinary Serum and Vaccine Research Institute, Cairo, Egypt..
Abstract
            Mycobacterium bovis (M. bovis), the cause of bovine tuberculosis (BTB) in cattle, remains a major zoonotic and economic problem in many countries. This study aimed to apply different serological tests for detection of bovine tuberculosis as a complementary for intradermal tuberculin test. The tested 4250 cattle in different Egyptian governorates by single intradermal cervical comparative tuberculin test (SICCT), 90 (2.1%) cattle reacted positively. The postmortem (PM) examination of the positive reactors revealed 65 (72.2%) out of 90 slaughtered cattle with visible lesions (VL) while the other 25 (27.8%) reactors showed no visible lesions (NVL). The visible lesions detected in head were 10 (15.4%) out of the 65 VL, in respiratory system were 32 (49.2%), in the digestive system were 5 (7.7%), in mixed infection were 11 (16.9%), and in generalized infection were 7(10.8%). The bacteriological examination of processed samples from the 90 slaughtered cattle revealed 55 M. bovis (61.1%); 51 (78.5%) were from 65 VL and 4 (16.0%) were from 25 NVL. Enzyme linked immune sorbent assay (ELISA) was used for the tuberculin positive animals’ sera and gave 36 (55.4%) out of 65 cattle with and 2 (8%) out of 25 cattle with NVL by a total 38 (42.2%) out of the 90 tuberculin positive animals. The results of lateral flow test gave 32 (49.2%) out of 65 cattle with VL and 1 (4%) out of 25 cattle with NVL by a total 33 (36.7%) out of the 90 tuberculin positive animals.
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