GENOMIC RELATEDNESS OF TURKEY CORONAVIRUSES, BOVINE CORONAVIRUS AND INFECTIOUS BRONCHITIS VIRUS OF CHICKENS | ||
| Kafrelsheikh Veterinary Medical Journal | ||
| Article 8, Volume 4, Issue 2, October 2006, Pages 105-118 PDF (602.09 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/kvmj.2006.114218 | ||
| Authors | ||
| M. M. Ismail* 1; A. Y. Metwally2 | ||
| 1Dept. of Poultry and Fish, Faculty of Veterinary Medicine, Kafrelsheikh University | ||
| 2Animal Health Research Institute, Kafr El-Sheikh Prov. Laboratory | ||
| Abstract | ||
| The discrepant findings of antigenic relationship of turkey coronavirus (TCV) to infectious bronchitis virus (IBV) and bovine coronavirus (BCV) lead us to study the genomic relatedness of TCVs to IBV and BCV. A one step reverse transcription (RT)-polymerase chain reaction (PCR) targeting a 525-base pair fragment of the spike 2 (S2) gene of BCV was used in a trial to detect TCVs isolated from diarrheic turkeys and a prototype TCV obtained from the American Type Culture Collection. The one-step RT-PCR amplified cell culture-passaged isolates of calf diarrhea strains of BCV, but none of the tested TCVs. On the other hand when we used primers designed from S2 gene of IBV, The same TCVs could be detected using the S2 IBV primers of IBV of chickens which amplified a 820-base pair region spanning portions of the S2 glycoprotein genes of IBV and TCV. These findings provide evidence that TCV is more closely related to IBV. | ||
| Keywords | ||
| turkey coronavirus; bovine coronavirus; infectious bronchitis virus; genomic relatedness | ||
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