PRODUCTION AND CHARACTERIZATION OF ANTIMICROBIAL ACTIVE COMPOUNDS FROM THE CYANOBACTERIUM NOSTOC COMMUNE VAUCH | ||
| Egyptian Journal of Phycology | ||
| Article 6, Volume 13, Issue 1, 2012, Pages 107-119 PDF (533.01 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/egyjs.2012.114967 | ||
| Authors | ||
| M. S. Abdel Hameed1, 2; S. H. Hassan* 1, 3; R. Abdel Salam4; R. Gamal1 | ||
| 1Botany Department, Faculty of Science, Beni-Suef University, Egypt | ||
| 2Biology Department, Faculty of Science, Jazan University, KSA | ||
| 3Medical laboratory Science Department, College of Applied Medical Science, Al Jouf University, KSA | ||
| 4Pharmacognosy Department, Faculty of Pharmacy, Beni Suef University, Egypt | ||
| Abstract | ||
| In this investigation, antimicrobial activity of Nostoc commune Vauch(isolated from agricultural wastewater canal, Beni Suef Governorate, Egypt) organic extracts were examined against nine selected microbial isolates. Four of them were Gram positive bacterial isolates (Bacillus subtilis, Mycobacterium phlei, Sarcina maxima andStaphylococcus aureus), four Gram negative bacteria (Escherichia coli, Proteus mirabilis,Pseudomonas aeruginosa and Salmonella arizonae) and one unicellular fungus (Candida albicans) were evaluated for their resistance against these extracts. Methanol was the best organic solvent for extraction of active material, rather than the other organic solvents. This material was produced, maximally, after 10 days of incubation in aerated shaken culture at 30ºC and pH 8.0 when N. commune was grown in Medium 18 growth medium. The antagonistic material was purified using thin layer chromatography then identified using chromatographic and spectroscopic techniques including UV, FT-IR, mass spectrophotometer and proton-NMR. Four unknown compounds were extracted that had long chain alcohol, sterol, long chain fatty acid and triterpen. These compounds were tested for their antimicrobial activity against B. subtilis. Only the long chain fatty acid (compound C) had an inhibitory effect on the growth of B. subtilis while the other compounds were not active. | ||
| Keywords | ||
| Nostoc commune; antimicrobial activity; organic extract | ||
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