Nasr, M., Hassan, E., Hassan, G., Ibrahim, A., Mohamed, S., Abdel-Aziz, A. (2019). Molecular screening of Helicobacter pylori among Egyptian patients with decompensated liver cirrhosis. test, 5(4), 533-542. doi: 10.21608/jbaar.2019.115570
Mohamed Y. Nasr; Ehsan Hassan; Ghada Hassan; Asmaa Ibrahim; Salwa. E. Mohamed; Amal Abdel-Aziz. "Molecular screening of Helicobacter pylori among Egyptian patients with decompensated liver cirrhosis". test, 5, 4, 2019, 533-542. doi: 10.21608/jbaar.2019.115570
Nasr, M., Hassan, E., Hassan, G., Ibrahim, A., Mohamed, S., Abdel-Aziz, A. (2019). 'Molecular screening of Helicobacter pylori among Egyptian patients with decompensated liver cirrhosis', test, 5(4), pp. 533-542. doi: 10.21608/jbaar.2019.115570
Nasr, M., Hassan, E., Hassan, G., Ibrahim, A., Mohamed, S., Abdel-Aziz, A. Molecular screening of Helicobacter pylori among Egyptian patients with decompensated liver cirrhosis. test, 2019; 5(4): 533-542. doi: 10.21608/jbaar.2019.115570

Molecular screening of Helicobacter pylori among Egyptian patients with decompensated liver cirrhosis

Journal of Bioscience and Applied Research
Article 11, Volume 5, Issue 4, October 2019, Pages 533-542    PDF (1.12 M)
Document Type: Original Article
DOI: 10.21608/jbaar.2019.115570
Authors
Mohamed Y. Nasr1; Ehsan Hassan2; Ghada Hassan1; Asmaa Ibrahim* 3; Salwa. E. Mohamed1; Amal Abdel-Aziz1
1Genetic Engineering and Biotechnology Research Institute, University of Sadat City (GEBRI, USC), Egypt
2National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt
3Diagnostic and Research Unit of Parasitic Diseases (DRUP), Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Background: Globally Helicobacter Pylori (H. Pylori) infection and liver diseases are renowned with their high incidence rate. Recent studies have observed the relation between H. Pylori infection and liver disease progression to cirrhosis. This study aimed to determine the prevalence of H. pylori in liver cirrhotic patients. Patient and Methods: This study was conducted on 100 liver cirrhotic patients to detect H. Pylori infection based on UreA gene detection in ascites using nested polymerase chain reaction (nPCR). Biochemical analysis was performed on ascitic fluid, serum, complete blood count (CBC), and polymorph nuclear leukocytes count (PMN). Result: H. Pylori infection was detected in 75% of patients with liver cirrhosis. In ascitic fluid, H. pylori infection showed a highly significant association with albumin and PMN (P=0.0001) and also with other biochemical analyses including serum ascites albumin gradient (SAAG), specific gravity, and glucose (P=0.005, 0.0031, and 0.02, respectively). In serum only, the infection showed significant association with alkaline phosphatase, albumin, and hemoglobin with P values of 0.008, 0.014, and 0.008, respectively. Conclusion: The current study results shed light on the significant role of H. Pylori infection in ascites of patients with decompensated end-stage liver cirrhosis suggesting a prospective relation between H. pylori infection and liver diseases progression due to hepatic infections especially HCV. 
Keywords
H. pylori; Nested PCR; UreA gene; Liver cirrhosis; HCV
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