Purification and Characterization of Alpha Toxin Clostridium perfringens Type A | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Article 6, Volume 12, Issue 2, December 2020, Pages 79-86 PDF (850.75 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2020.115949 | ||
| Authors | ||
| zahra heshmati1; Mohsen Fathi Najafi2; Gholam hossein Ronaghi1 | ||
| 1Department of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran | ||
| 2Education and Extension Organization (AREEO), Agricultural Research, Razi Vaccine and Serum Research Institute, Mashhad Branch, Mashhad, Iran | ||
| Abstract | ||
| Clostridium perfringens is a toxin-producing anaerobe with the ability to cause multiple diseases in humans and animals. One of the toxins produced by all strains of this bacterium is an alpha toxin (phospholipase C). This study aimed to purify and characterize alpha-toxin. C. perfringens type A was grown in an optimized medium, the culture supernatant was separated, and ammonium sulfate precipitated protein was subjected to ion exchange and gel filtration chromatography methods. The purification steps were monitored by SDS-PAGE and hemolysin activity for each sample and performed by the hemolysin assay method. The purified alpha-toxin appeared in a single band in 43 KDa. The enzymatic efficiency yield was about 88% with a specific activity of 69170 and 654 fold of purification. According to the results, alpha-toxin is Zn and Mg metal-enzyme dependent on the temperature and pH optimum of 40 °C and 6, respectively. The enzyme Km and Vmax values of 3.8 mg/ml and 0.19 were calculated as the enzyme in the presence of lecithin. The partially purified alpha toxin with specific characters can be used for different applications such as antibody production and determination of the anti-toxin rate in animal serum. | ||
| Keywords | ||
| Clostridium Perfringense; alpha toxin; chromatography; lecithinase; characterization | ||
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