Antibacterial activity of phenolic extract of Spirulina platensis and its structural elucidation of bioactive compound | ||
| Egyptian Journal of Phycology | ||
| Article 3, Volume 18, Issue 1, 2017, Pages 45-57 PDF (480.46 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/egyjs.2017.115987 | ||
| Authors | ||
| Hind M. A. El-Sayed* 1; Mohammed Rawway2; Nermine N. Abed3; Rawhya A. Salah El Din3 | ||
| 1Medical analysis specialist | ||
| 2Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Assiut) | ||
| 3Botany and Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch) | ||
| Abstract | ||
| Spirulina as many other cyanobacteria species have the potential to produce a large number of antimicrobial substances, so they are considered as suitable organisms for exploitation as biocontrol agents of pathogenic bacteria. In the present study, antimicrobial activity of Spirulina platensis phenolic acid extract was investigated against pathogenic bacteria which cause sepsis in neonates. The algal extract was tested in vitro for their antibacterial effect against (Bacillus cereus, Enterococcus casseliflavus, Kocuria kristinae, Staphylococcus aureus, Alloiococcus otitis, Rosemonas gilardii,and Aeromonas hydrophila) using paper disc diffusion method and observed all these bacteria showed inhibition in growth by this extract. The bioactive components of Spirulina platensis have been assessed using GC-MS analysis. Eleven compounds in phenolic acid extract of the alga were identified. Bis(2-ethylhexyl) Phthatate (25.899%), Palmitic acid (14.263%) ,were the most prevailing compounds in phenolic acid extract. | ||
| Keywords | ||
| Spirulina platensis; antibacterial activity; sepsis; paper-disc diffusion method; Gas chromatography-mass spectrometry (GC-MS) analysis | ||
| References | ||
|
Alam, M.A.; Haider, N.; Ahmed, S.; Alam, M.T.; Azeez, A. and Perveen, A. (2013). Tahlab (Spirulina) And Few other Medicinal Plants Having Anti-Oxidant & Immunomodulatory Properties Described In Unani Medicine-A Review. Int. J. Pharm. Sci. Res., 4: 4158-4164.
Al-Marzoqi, A.H.; Hameed, I.H. and Idan, S. A. (2015). Analysis of bioactive chemical components of two medicinal plants (Coriandrum sativum and Melia azedarach) leaves using gas chromatography-mass spectrometry (GC-MS).Afr. J. Biotechnol., 14:2812-2830.
Asghari, A.; Fazilati1, M.; Latifi, A.M. Salavati, H.and Choopani, A. (2016).A Review on Antioxidant Properties of Spirulina. Journal of Applied Biotechnology Reports, 3 (1): 345-351.
Austin, B., 1992. Inhibition of bacteria fish pathogens by Tetrasel missuecica.J. Fish Diseas., 15: 53-61.
Bauer, B.E. (1996). Amino- and carboxy-terminal domains of the yeast Rab escort protein are both required for binding of Y pt small G proteins. Mol. Biol. Cell.7:1521-33.
Black, J.G. (1999). "Microbiology": Principles and Explorations Marymount University.Wiley in New York.
Bloor, S. and England, R.R. (1991). Elucidation and optimization of the medium constituents controlling antibiotic production by the cyanobacterium Nostoc muscorum.Enzyme Microb. Technol., 13: 76-81.
Cerone, S.I.; Sansinanea, A.S. and García, M.C. (2007). Effects of beta-hydroxybutyric acid on bovine milk leukocytes function in vitro.Gen. Physiology Biophys., 26: 14-19.
Dehnen, W.; Pitz, R. and Tomingas, R. (1977). The mutagenicity of airborne particulate pollutants. Cancer Lett., 4: 5-12.
Demule, M.C.Z.; Decaire, G. Z. and Decano, M. S. (1996). Bioactive substances from Spirulina platensis. Int. J. Exp. Biol., 58: 93-96.
Edwards, M.S. and Gonik, B. (2013). Preventing the broad spectrum of perinatal morbidity and mortality through group B streptococcal vaccination. Vaccine. 31: 66-71.
Feigin, R.D. and Cherry, J.D. (1992).Text Book of Pediatric Infectious Disease. Saunders , Philadelphia, 110 – 15.
Gourlay, T.; Samartzis, I. ; Stefanou, D. and Taylor, K. (2003). Inflammatory response of rat and human neutrophils exposed to di-(2-ethyl-hexyl)-phthalate plasticized polyvinyl chloride.Artificial Organs. 27: 256-260.
Gopalakrishnan, S. and Vadivel, E. (2011). GC-MS Analysis of Some Bioactive Constituents of Mussaenda frondosa Linn. International J. Pharm. and Bio Sciences. 2:313-320.
Hashimoto, K.; Urakami, K.; Fujiwara, Y.; Terada, S. and Watanabe, C. (2001). Determination of residual solvents in pharmaceuticals by thermal desorption-GC/MS, Analyt. Sci., 17: 645–648.
Hornik, C. P.; Fort. P. and Clark, R. H. (2012). Early and late onset sepsis in very-low-birth-weight infants from a large group of neonatal intensive care units. Early Human Develop., 88: 69-74.
Ishida, K.; Matsuda, H.; Murakami, M. and Yamaguchi, K. (1997). Kawaguchipeptin B. and antibacterial cyclic undecapeptide from the cyanobacterium Microcystis aeruginosa.J. Nat. Prod., 60: 724-726.
Ismail, M.; Hossain, M.F.; Tanu, A.R. and Shekhar, H. U. (2015). Effect of spirulina intervention on oxidative stress, antioxidant status, and lipid profile in chronic obstructive pulmonary disease patients. BioMed. Res. Int., 7-15.
Karambin, M. and Zarkesh, M. (2011). Entrobacter the most common pathogen of neonatal septicemia in Rasht, Iran. Iran J. Pediatr., 21: 83-7.
Kaushik, P. and Chauhan, A. (2008). In vitroantibacterial activity of laboratory grown culture of Spirulina platensis. Ind. J. Med. Microbiol., 48: 348-52.
Kornhuber, J.; Muehlbacher, M.; Trapp, S.; Pechmann, S.; Friedl, A.; Reichel, M.; Mühle. C.; Terfloth, L.; Groemer, T.; Spitzer G.; Liedl, K.; Gulbins, E. and Tripal, P. (2011). "Identification of Novel Functional Inhibitors of Acid Sphingomyelinase". PLoS ONE. 6 (8): e23852.
Kumar, P.; Angadi, S. and Vidyasagar, G. (2006). Antimicrobial activity of blue green and green algae.Ind. J. Pharm. Sci., 68: 647-648.
Lee, K. H.; Kim, J. H. ; Lim, D. S. and Kim, C. H. (2000). Anti-leukemic and anti-mutagenic effects of di-(2-ethylhexyl) phthalate isolated from Aloe vera Linne. J. Pharm. Pharmacol., 52: 593-598.
Lehn, J. M. and Weinheim, V.C.H. (1995). "Supramolecular Chemistry Concepts and Perspectives". BRIDGES, Collège de France, 11, Place Marcelin Berthelot, Paris.
Markowitz, J. S.; Straughn, A. B. and Patrick, K. S. (2003). "Advances in the pharmacotherapy of attention-deficit-hyperactivity disorder: focus on methylphenidate formulations".Pharmacotherapy. 23 : 1281–99.
Mattila, J.; Omelyanchuk, L.; Kyttala, S.; Turunen, H. and Nokkala, S. (2005). Role of Jun N-terminal Kinase (JNK) signaling in the wound healing and regeneration of a Drosophila melanogaster wing imaginal disc. HYPERLINK "http://dx.doi.org/10.1387/ijdb.052006jm"Int. J. Develop. Biol.HYPERLINK "http://dx.doi.org/10.1387/ijdb.052006jm", 49: 391HYPERLINK "http://dx.doi.org/10.1387/ijdb.052006jm"-399.
McGaw, L. J.; Jäger, A.K. and Van Staden, J. (2002). Isolation of antibacterial fatty acids from Schotia brachypetala. Fitoter., 73:431-433.
Oie, L.; Hersoug, L.G. and Madsen, J. O. (1997). Residential exposure to plasticizers and its possible role in the pathogenesis of asthma. Environ.Health Perspect., 105: 972-978.
Parages, M. L.; Rico, R. M.; Abdala-Díaz, R. T.; Chabrillón, M.; Sotiroudis, T. G. and Jiménez, C. (2012). Acidic polysaccharides of Arthrospira (Spirulina) platensis induce the synthesis of TNF- α in RAW macrophages. J. Appl. Phycol., 24: 1537-1546.
Paula, J. A. M.; Silva, M. R. R.andCosta, M. P. (2012). Evidence-Based Complementary and Alternative Medicine. 420715. Peer-reviewed journal,Hindawi Publishing Corporation.
Pyne, S.K.; Bhattacharjee, P. and Srivastav, P.P. (2017). Microalgae (Spirulina platensis) and Its Bioactive Molecules: Review.Indian Journal of Nutrition, 4 (2): 2395-2326.
Rad, Z.A.; Mojaveri , M. H.; Esmaeilzadeh, S.; Firouzjahi, A.; Laegh, M.; Khafri, S. and Rad, M. N. (2016).Colonization of rectovaginal Escherichia coli and group B streptococci in mothers and on infants' body surface and their related risk factors. Caspian Journal of Pediatrics, 2 (2): 148-52.
Riezzo, I.; Centini, F.; Neri, M.; Rossi. G.; Spanoudaki, E.; Turillazzi, E. and Fineschi, V. (2009). "Brugada-like EKG pattern and myocardial effects in a chronic propofol abuser".Clin. Toxicol., 47: 358-63.
Rowshanul, M. and Rezaul, M. (2009). Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol- 3-acetate Isolated from Calotropis gigantea (Linn.) flower.Mycobiol., 37: 31-36.
Schuchat, A. (2000). Neonatal group B streptococcal diseasescreening and prevention. New Eng. J. Med., 343: 209-10.
Siddabathuni, A.; Akalanka, D. and Somnath, D. (2014). Gas Chromatography-Mass Spectrometry Analysis of Kyllinga Triceps.IJPSR, 5: 2999-3003.
Tuney, I.; Cadirci, B. H.; Unal, D. and Sukatar, A. (2006). Antimicrobial activities of the extracts of marine algae from the coast of Urla (Izmir, Turkey).Turk. J. Biol., 30: 171-175.
Vinay, A. K.; Bhatnagar, J.; Srivastava, J. N. (2011). Antibacterial activity of crude extracts of Spirulina platensis and its structural elucidation of bioactive compound. J. Med.l Plants Res., 5:7043-7048. | ||
|
Statistics Article View: 269 PDF Download: 294 |
||
