DNA polymorphism of FSHR gene and its association with infertility traits in Egyptian buffaloes | ||
| Benha Veterinary Medical Journal | ||
| Article 1, Volume 39, Issue 1, September 2020, Pages 1-4 PDF (158.22 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2020.34139.1221 | ||
| Authors | ||
| Sherif Ramadan* 1; Abdel-Aziz Saker2; Basant Shafik1 | ||
| 1Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Egypt | ||
| 2Department of biotechnology, Animal production Research institute, Agriculture Research Center, Cairo, Egypt | ||
| Abstract | ||
| The objectives of this study were to detect genetic polymorphism in exon 10 of Follicle Stimulating Hormone Receptor gene (FSHR) by using RFLP and direct sequence methods, also to investigate their possible association with infertility traits in Egyptian buffaloes. A total number of 50 female Egyptian buffaloes at Mahallet Mousa experimental farms in Kafr El-sheikh province were investigated. Animals were divided into three groups: normal fertile (20 animals), infertile due to anestrum (15 animals) and infertile due to repeat breeding (15 animals). Genomic DNA was extracted from blood samples, then PCR was performed with annealing temperatures of 57ºC to amplify 231 bp of FSHR exon 10 gene. The amplicons were digested with Hin1II (NlaIII) restriction enzyme. The results showed that all the investigated buffaloes were monomorphic and genotyped as A/A genotype. Moreover, the monomorphism of buffaloes FSHR exon 10 gene was confirmed by sequencing three samples from each buffaloes’ group. It seems that FSHR gene are highly conserved in the investigated buffalo’s population. | ||
| Keywords | ||
| Egyptian buffaloe; fertility; FSHR; Polymorphism; RFLP | ||
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