Desouky, A., Saad, A., Elshorbagy, M., Abodalal, S. (2020). Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate. test, 39(1), 159-164. doi: 10.21608/bvmj.2020.32932.1216
Asmaa Ibrahim Desouky; Ahmed Eissa Mohamed Saad; Mohamed Abdelgide Elshorbagy; Samah Abodalal. "Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate". test, 39, 1, 2020, 159-164. doi: 10.21608/bvmj.2020.32932.1216
Desouky, A., Saad, A., Elshorbagy, M., Abodalal, S. (2020). 'Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate', test, 39(1), pp. 159-164. doi: 10.21608/bvmj.2020.32932.1216
Desouky, A., Saad, A., Elshorbagy, M., Abodalal, S. Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate. test, 2020; 39(1): 159-164. doi: 10.21608/bvmj.2020.32932.1216

Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate

Benha Veterinary Medical Journal
Article 30, Volume 39, Issue 1, September 2020, Pages 159-164    PDF (698.68 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2020.32932.1216
Authors
Asmaa Ibrahim Desouky* 1; Ahmed Eissa Mohamed Saad2; Mohamed Abdelgide Elshorbagy3; Samah Abodalal4
1Avain and Rabbit disease department-veterinary medicine-benha univerisity
2Avain and Rabbit disease department faculty of veterinary medicine benha university
3Avain and Rabbit Diseases department faculty of veterinary medicine benha university
4Newcastle disease department veterinary serum and Vaccine Researcher Institute, Agriculture Research Center, Abbasia, Cairo, Egypt
Abstract
A B S T R A C T
Newcastle disease (ND) is a highly contagious disease causing huge economic losses to the poultry industry due to high morbidities and mortalities. This study was aimed to isolate and characterize the new sub-genotype NDV strains in Qalybia Governorate during 2017 and 2019. Sixty eight samples were collected from Sixty eight broiler chicken flocks suffering from high mortality reach to 80% with distinctive clinical signs and post-mortem lesions of NDV. NDV strains isolated through inoculation in specific pathogen free (SPF) eggs and were identified by hemagglutination (HA) and hemagglutiation inhibition(HI) techniques. There were 16/20 (about 80%) of selected suspected farm were positive for NDV With HI test . These hemagglutinating agents were confirmed as NDV strains through molecular characterization using one step RT-PCR targeting the partial F-gene of NDV. Sequencing and phylogenetic analysis were successfully performed with eight NDV isolates which possessed the motif 112 R/K-R-Q-K/RR116 at the C-terminus of the F2 protein and F (phenylalanine) at residue 117, indicating that they are velogenic genotype. The present study revealed the circulation of NDV class II genotype VII in Qalybia chicken flocks.
Keywords
Key words: Newcastle Disease; F protein Cleavage site; Genotype VII
Statistics
Article View: 254
PDF Download: 371