Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt | ||
| Benha Veterinary Medical Journal | ||
| Article 32, Volume 39, Issue 1, September 2020, Pages 169-174 PDF (193.8 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2020.38777.1242 | ||
| Authors | ||
| Ibtehal Saeed Abdelaziz* 1; Ashraf Awad Abd El-Tawab2; Ahmed Afifi Maarouf3; Fatma Ibrahim Elhofy2 | ||
| 1Bacteriology. Veterinary Medicine Benha University. Egypt | ||
| 2Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt | ||
| 3Chief Researcher of Food Hygiene, Animal Health Research Institute, Benha Branch | ||
| Abstract | ||
| This study was conducted on 21 commercial duckling farms (1- 20 days old) inspected to show Salmonella infection in different localities at Kaliobia Governorate. Samples were taken from diseased ducklings and freshly dead for bacteriological examination which resulted in, 94 samples were positive from 630 isolates, where 28 isolates from 33 diseased ducklings and 66 isolates from 72 freshly dead ducklings. Three serogroups of Salmonella were obtained by serological identification (Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Blegdam). The antibiotic sensitivity tests for the isolated strains showed multiple antibiotic resistances (oxytetracycline; amoxicillin; ampicillin; streptomycin; erythromycin and trimethoprim/ sulphamethoxazol) but gentamycin, norfloxacin and ciprofloxacin are the most effective antibiotic on the isolated Salmonella and can be used for treatment of Salmonellosis in duck farms. PCR results appeared that, invA and stn genes were detected in all studied Salmonella isolates;pefA gene was detected in four out of five studied isolates but sefC genewas detected in two isolates only. Finally, isolated Salmonellae are virulant pathogens responsible for disease in ducklings resulting in high mortality and morbidity, gentamycin, norfloxacin and ciprofloxacin are the most proper antibiotics used for treatment of Salmonellosis in duck farms. | ||
| Keywords | ||
| Duckling; Salmonella; antibiotic resistant; virulence genes | ||
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