El-Din, H., Rashad, A., El – Khayat, E., Shams El–Din, A., Abd–Elzaher, T. (2020). Biochemical and Histological Effects of some Natural Plant Essential Oils on Pink Bollworm, Pectinophora gossypiella (Saund.) and Spiny Bollworm, Earias insulana (Boisd.) Larvae. test, 11(9), 455-459. doi: 10.21608/jppp.2020.117992
Hanady S. El-Din; Amira M. Rashad; E. F. El – Khayat; A. M. Shams El–Din; Tahany R. Abd–Elzaher. "Biochemical and Histological Effects of some Natural Plant Essential Oils on Pink Bollworm, Pectinophora gossypiella (Saund.) and Spiny Bollworm, Earias insulana (Boisd.) Larvae". test, 11, 9, 2020, 455-459. doi: 10.21608/jppp.2020.117992
El-Din, H., Rashad, A., El – Khayat, E., Shams El–Din, A., Abd–Elzaher, T. (2020). 'Biochemical and Histological Effects of some Natural Plant Essential Oils on Pink Bollworm, Pectinophora gossypiella (Saund.) and Spiny Bollworm, Earias insulana (Boisd.) Larvae', test, 11(9), pp. 455-459. doi: 10.21608/jppp.2020.117992
El-Din, H., Rashad, A., El – Khayat, E., Shams El–Din, A., Abd–Elzaher, T. Biochemical and Histological Effects of some Natural Plant Essential Oils on Pink Bollworm, Pectinophora gossypiella (Saund.) and Spiny Bollworm, Earias insulana (Boisd.) Larvae. test, 2020; 11(9): 455-459. doi: 10.21608/jppp.2020.117992

Biochemical and Histological Effects of some Natural Plant Essential Oils on Pink Bollworm, Pectinophora gossypiella (Saund.) and Spiny Bollworm, Earias insulana (Boisd.) Larvae

Journal of Plant Protection and Pathology
Article 4, Volume 11, Issue 9, September 2020, Pages 455-459    PDF (920.8 K)
Document Type: Original Article
DOI: 10.21608/jppp.2020.117992
Authors
Hanady S. El-Din* 1; Amira M. Rashad1; E. F. El – Khayat2; A. M. Shams El–Din2; Tahany R. Abd–Elzaher2
1Bollworms Dept., Plant Prot. Res. Inst., A. R. C., Dokki, Giza
2Dept. of Econ. Entomol., Fac. of Agric., Benha Univ., Egypt
Abstract
Biochemical and histological studies were executed on 4th larval instar larvae of the Pink bollworm (PBW) and the spiny bollworm (SBW) treated as newly hatched larvae with LC50 for two tested oils (Jojoba oil, and flaxseed oil). Results noticed that Jojoba oil and flaxseed oil decreased protein levels in Pectinophora gossypiella by 35.92 and 20.73% as compared with control, respectively. On the other hand, they caused increases in protein levels in Earias insulana, by 7.93% for Jojoba and by 4.50% for Flax. The total soluble lipids were estimated by -44.82and 45.86%, respectively for P. gossypiella, while they were -5.26 % in Jojoba and -11.48 in Flaxseed oil for E. insulana. Carbohydrate hydrolyzing enzymes activity was clearly increased by both treatments on E. insulana, while variations impacts on P. gossypiella were recorded (-34.19 % for Jojoba oil and 6.95% for Flaxseed oil). General increases in GOT and GPT transaminaes activity were recorded in P. gossypiella and E. insulana by due to the used oil treatments, but reduction percentages were found in alkaline phosphates as well as for Lactate dehydrogenase catalyzes (LDH) when the investigated pests treated with Jojoba and Flaxseed oils. Also, the histopathological disturbance was estimated in this study when neonate larvae fed on diet treated with LC50 value of Jojoba oil and flaxseed oil. The cuticle and mid-gut tissues of the 4th instar larvae showed vacuolization with hypertrophied lining mucosal epithelial, and the epithelial cells are blurred and Necrosis of nuclei.
Keywords
Pectinophora gossypiella, PBW; Earias insulana, SBW; Jojoba oil, Flaxseed oil, biochemical studies and histopathological studies
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