FACTORS AFFECTING EMBRYOGENESIS IN MICROSPORE CULTURES OF BROCCOLI (Brassica oleracea var. italica) | ||
| Journal of Plant Production | ||
| Article 25, Volume 34, Issue 5, May 2009, Pages 5067-5076 PDF (817.46 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2009.118384 | ||
| Authors | ||
| M. A. Badawi1; E. E. Metwally2; Sahar S. Taha1; Marwa O. Arafeh1 | ||
| 1Department of Vegetable Crops , Faculty of Agric., Cairo Univ. , Egypt. | ||
| 2Department of Vegetable Crops , Faculty of Agric., Tanta Univ. , Egypt | ||
| Abstract | ||
| Microspore culture is a very important and useful tool in plant breeding for haploid production and has been developed for many years. Broccoli (Brassica oleracea var. italica) is an important vegetable crop. Conditions for reliable induction of embryogenesis from isolated microspores were studied in eight genotypes of broccoli (Hanin, Conde F1, Baladi, Belstar F1, Marathon F1, Parthenon F1, Naxos F1 and Tiburom ). The optimum timing for microspore culture was confirmed to be during the mid to late uninucleate stage. The highest yield was 237.33 embryo per dish from genotype Parthenon F1. Embryo yields were significantly increased in broccoli genotypes by the incubation at 32.5 ◦C for 24 hours, than that incubated at 32.5 ◦C for 48 hours or 35.5 ◦C for 24 or 48 hours. The use of the 1/2 NLN-13 medium yielded greater number of embryos than the standard NLN-13 and B5 media. The magnitude of the response to the reduction of the concentration of major salts by half in the NLN medium varied with the different genotypes. Parthenon F1 and Marathon F1 presented a better response to the reduction of the concentration of major salts by half in NLN-13. Microspore culture density on embryo production was evaluated in selected genotypes. Microspore plating density was critical for efficient embryonic induction and development, with an optimal plating density of 4×104 microspore/ml. which obtained resulted in 237.33 embryo from Parthenon F1 and 171.00 embryo from Marathon F1. Activated charcoal (0.2 ml) added to the liquid NLN-13 medium, increased embryo yield significantly as compared to those cultures without activated charcoal. | ||
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