Role of Some Fatty Acids in the Dynamics of Follicular Fluid in She- Camel | ||
| Egyptian Journal of Biomedical Engineering and Biophysics | ||
| Article 2, Volume 12, Issue 1, December 2011, Pages 21-31 PDF (814.62 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejbbe.2011.1184 | ||
| Abstract | ||
| THE MAIN objective of this work was to determine the activity of some …..antioxidants and content of fatty acid in the follicular fluid in relation to size of the follicle and quality of oocyte in she camels (Camelus dromedaries). The vesicular follicles (≥ 3mm in diameter) were selected from the obtained ovaries, and classified according to their surface diameter into small, medium, large and very large size follicles (3-5, > 5-10, > 10-15 and > 15mm, respectively). The aspirated follicular fluid was centrifuged to separate cell fraction and then examined for oocyte quality. Most of the recovered small and medium size follicles were of good quality (69.23 and 59.19%, respectively) in comparison to those recovered from large and very large ones (33.33 and 23.08% , respectively ). The activity of SOD (superoxide dismutase) was elevated and those of GSH (glutathione) dropped in the follicular fluid as the diameter of follicles increased and the percentage of oocytes with good quality was decreased. Palmitic (C16:0) and oleic (C18:1) acids were the most abundant fatty acids in the follicular fluid, while, those of stearic (C18:0), linoleic (C18:2) and arachidonic (C20:4) acids were lower. Other fatty acids were very low. Moreover, the content of stearic (C18:0), oleic (C18:1), linoleum (C18:2) and arachidonic (C20:4) acids in the follicular fluid declined as the diameter of follicles was increased and the percentage of oocytes with good quality decreased. Those of other fatty acids did not vary significantly with the change in the size of follicle and /or quality of oocytes. | ||
| Keywords | ||
| follicular fluid; oocytes; fatty acids | ||
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