Shaaban, H., Shabbara, H., Farag, M., El saadawi, W. (2016). A Simple Method to Obtain Microbial-free in vitro Moss Cultures. test, 36(1), 17-25. doi: 10.21608/taec.2016.11937
Hanan Shaaban; Hanaa Shabbara; Mohamed Farag; Wagieh El saadawi. "A Simple Method to Obtain Microbial-free in vitro Moss Cultures". test, 36, 1, 2016, 17-25. doi: 10.21608/taec.2016.11937
Shaaban, H., Shabbara, H., Farag, M., El saadawi, W. (2016). 'A Simple Method to Obtain Microbial-free in vitro Moss Cultures', test, 36(1), pp. 17-25. doi: 10.21608/taec.2016.11937
Shaaban, H., Shabbara, H., Farag, M., El saadawi, W. A Simple Method to Obtain Microbial-free in vitro Moss Cultures. test, 2016; 36(1): 17-25. doi: 10.21608/taec.2016.11937

A Simple Method to Obtain Microbial-free in vitro Moss Cultures

Taeckholmia
Article 2, Volume 36, Issue 1, 2016, Pages 17-25    PDF (666.03 K)
Document Type: Original Article
DOI: 10.21608/taec.2016.11937
Authors
Hanan Shaaban* ; Hanaa Shabbara; Mohamed Farag; Wagieh El saadawi
Botany Department, Faculty of Science, Ain Shams University, Abbasia, Cairo, Egypt.
Abstract
Developing a suitable method for moss sterilization is a crucial step for successful in
vitro culture. Different chemical and physical methods have been tried by many authors
for more than a century but with low axenic culture percentages. An easy and economic
(no use of chemicals or antibiotics) cultivation method is developed here, which proved
high success with three moss species starting from gametophores.
Keywords
Axenic cultivation; Mosses; Bryum; Philonotis; sterilizing agents
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