Direct Effects of Temperature Changes on Biochemical and Enzymatic for Cotton Leafworm, Spodoptera littoralis (Boisd.) | ||
| Egyptian Academic Journal of Biological Sciences. A, Entomology | ||
| Article 12, Volume 11, Issue 1, February 2018, Pages 121-136 PDF (749.93 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajb.2018.11985 | ||
| Authors | ||
| Aida, S. Kamel1; Walaa, E. Gamil2; Dahi H. F.2 | ||
| 1Entomology Department, Faculty of Science, Benha University, Egypt | ||
| 2Plant Protection Research Institute, Agricultural Research Centre, Giza, Egypt | ||
| Abstract | ||
| It is important to understand the effects of increasing temperatures due to global warming on Egyptian cotton leafworm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) which damages a wide variety of crops in the Middle East and is one of the major economic pests of cotton in Egypt. Therefore,we are investigating five different constant temperatures at 15, 20, 25, 30and 35oC as different thermal effects on biochemical parameters and enzymes of fourth larval instars. Biochemical analyses were performed to determine the effects of these different constant temperatures on the amount of total proteins, lipids, carbohydrates and free amino acids as well as metabolic, digestive, neural and detoxification enzymes. It's clear from the results that the total amount of proteins, free amino acids as well as, the activity levels of proteases were increased significantly by raising of temperatures to reach their maximum activities at 30oC. While the total amount of lipids, Carbohydrates were decreased significantly by raising of temperatures. Moreover, activity levels of amylase, invertase and Trehalase were decreased at 15oC.Amylase and invertase reached their maximum activity at 30oC,while Trehalase reached its maximum activity at 20oC. There was severe reduction in carbohydrate enzymes activities at 35oC. On other hands, the activity levels of total lipids and lipase were increased significantly at lower temperatures 15oC and 20oC, no significant changes were detected between other temperatures. The activity levels of GOT and GPT increased significantly and reached their maximum level at 20oC.However, GOT and GPT at other temperature were significantly different. The level of GST Enzyme was increased significantly at 30oC, while, its activity level was decreased at 15oC. Moreover, AchE, Alpha (EST-α) and beta esterase(EST-β) enzymes were increased gradually at 15,20 and 25 oC. They reached their maximum activities at 25oC followed by the reduction in activity levels at 30oC and 35oC. | ||
| Keywords | ||
| Temperature; Spodoptera littoralis; biochemical analyses; Metabolic; digestive; neural and detoxification enzymes | ||
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