Macro-, micromorphological characters and DNA fingerprinting markers on three Ruprechtia species (Polygonaceae) in Egypt | ||
| Taeckholmia | ||
| Article 1, Volume 21, Issue 1, 2001, Pages 1-13 PDF (423.21 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/taec.2001.12449 | ||
| Authors | ||
| Mohamed Tantawy* ; Maher Shehata | ||
| Botany Dept., Faculty of Science, Ain Shams University-Egypt. | ||
| Abstract | ||
| Macro-,micromorphological characters and DNA fingerprinting markers have been used to differentiate the three Ruprechtia C. A. Meyer species growing in Egypt (R. exelsa, R. polystachya, and R. salicifolia). Interestingly, the macro- and micromorphological criteria of the vegetative organs showed no fundamental characters to discriminate between the three examined species. However, the data obtained from the seed coat surface (using LM and SEM) revealed some variability among the tested species. Seed coat surface was colliculate in (R. excelsa), pusticulate in (R. polystachya) and reticulate–scalariform in R. salicifolia. Accordingly, the seed coat surface can be used as a fundamental criterion to discriminate between the species of this study. The RAPD– PCR electrophoretic profile, showed unique RAPD markers some of which were species-specific. Speciesspecific markers were recorded by using primers OPA-01, OPA-02 and OPA-05 respectively. Two RAPD markers specific to R. polystachya and R. salicifolia were recorded. The two markers for the former species have a molecular size of 1800 and 2600 bp using primer OPA-01. While those specific to the latter species have a molecular size of 300 and 400 bp using primers OPA-02. For R. excelsa, one specific RAPD marker was recorded, it has a molecular size 800 bp using primer OPA-05. The RAPD markers may be considered as valid criteria to discriminate between the three Ruprechtia species used in this investigation. However, the similarity in the banding profile of RAPD-PCR and the morphological criteria justify the maintenance of the three species in the same genus. | ||
| Keywords | ||
| Ruprechtia; Polygonaceae; Morphology; Seed coat surface; DNA fingerprinting | ||
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