THE ROLE OF THE BACTERIUM PASTEURIA PENETRANS, SOME SOIL AMENDMENTS AND MARIGOLD PLANT EXTRACT IN CONTROLLING ROOT-KNOT NEMATODE. | ||
| Menoufia Journal of Plant Protection | ||
| Article 12, Volume 2, Issue 3, May and June 2017, Pages 261-262 PDF (213.41 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjapam.2017.125983 | ||
| Authors | ||
| E. M. Mousa1; M. E. Mahdy1; A. M. Kella2 | ||
| 1Prof. of Plant Pathology, Fac. of Agric., Menoufia Univ. | ||
| 2Prof. of Nematology, Plant Pathology Research Institute, A.R. Center | ||
| Abstract | ||
| In this study the effect of different temperature degrees i.e 5, 10, 20, 30 and 35oC on the attachment process of Pasteuria penetrans spores and larvae of Meloidogyne spp. after 2, 12 and 24 hrs. were studied In Vitro. The highest attachment recorded after 12hrs at 30oC. The effect of the different soil types were also studied In Vitro. Swedge sludge was also used at 5 types i.e. swedge sludge alone; swedge sludge mixed with hay bean and rice straw at 1:2:1; swedge sludge mixed with hay bean at 1:1; swedge sludge mixed with hay bean, rice straw at 1:1:1; swedge sludge mixed with rice straw at 1:1 and applied at the rate of 5, 10, 15, 20, 30 and 50 g/pot. Compost was also used at the same concentration to control root-knot nematodes on tomato plants. Results revealed that the highest reduction in nematode parameters recorded with swedge sludge mixed with hay bean 1:1 at 5 g/pot. Marigold plants were also used at different application types i.e. fresh and powder of flowers, shoots and roots at 5, 10 and 20g/pot as well as the whole marigold plant after its dipping in water for 1 and 2 hours at the same concentration 5, 10 and 20 g/pot. Pots were irrigated by 10 ml of the water that used in dipping process around the roots. Results revealed that fresh flowers application was the best one in reducing nematode parameters at all used concentration. Two isolates of Pasteuria penetrans namely Egyptian and Japanese isolates were used at four application forms: spore suspension, Pp infected root powder; Pp infested soil and Pp infected females. Results revealed that application the Japanese isolate at Pp infected roots powder and the Egyptian one also as a root powder were the best treatments in reducing the nematode parameters. | ||
| Keywords | ||
| Meloidogyne; Pasteuria penetrans; swedge sludge; Compost; marigold | ||
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