CIRCULATING PATTERN OF RESPIRATORY VIRUSES, IN EGYPT, SEASON 2013-2014 | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Article 14, Volume 43, Issue 2, December 2020, Pages 237-253 PDF (588.74 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2020.127419 | ||
| Authors | ||
| Wael H. Roshdy1; Amel M. Naguib2; Ibrahim Elsayed3; Yara I. Shamikh4 | ||
| 1PhD in Biochemistry and Molecular Virology, Head of Tissue Culture and Virus Isolation, Central Public Health Laboratories, CPHL, Ministry of Health, Cairo 11613, Egypt | ||
| 2MSc in Clinical Pathology, Head of Virology Directorate, Central Public Health Laboratories, CPHL, Ministry of Health, Cairo 11613, Egypt | ||
| 3Department of Biochemistry, Faculty of Science, Kafr Elsheikh University, Kafr Elsheikh 33784, Egypt | ||
| 4Department of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt & Consultant, Virology Department, Egypt Centre for Research and Regenerative Medicine, ECRRM, Cairo 11517, Egypt | ||
| Abstract | ||
| Respiratory viruses causes lower and upper respiratory tract infections; LRTIs and URTIs. These infections are from the predominant causes of infections, especially in children and infants. Objective: This study, provides the evidence of using modern techniques as the multiplex PCR method, as a helpful tool in identifying the circulating pattern of respiratory viruses, in Egypt, thus this could help in identifying and inventing new treatment and vaccines. Subjects and methods: A number of 237 nasopharyngeal aspirates, were collected between December 2013 to November 2014, from 2 Egyptian fever hospitals, classified into 3 groups; group 1; 37 normal persons, group 2; 100 patients with LRTIs or pneumatic group and group 3; 100 patients with URTIs or common cold group. The results were then assayed using multiplex RT-PCR, in the Central Public Health Laboratories, CPHL, Ministry of Health, Cairo, Egypt. Results: Respiratory viruses, were detected in 152 cases, where the human rhinovirus was the most prominent; 15.6%, followed by the respiratory syncytial virus, 10.5%, metapneumovirus; 13%, influenza-A (Flu-A); 6.3%, adenovirus (AdV); 5.9%, influenza-B (Flu-B); 5.4%, parainfluenza-3 (PIV-3); 4% and a percentage of 0.42% infection for parainfluenza-1 (PIV-1), parainfluenza-2 (PIV-2) and human bocavirus (HBoV). No infections with parainfluenza-4, enterovirus nor the coronaviruses were detected. Both season and age of the patients, affect the detection rate of the analysis. | ||
|
Statistics Article View: 376 PDF Download: 458 |
||
