Goel, H., Singla, R., Chawla, R., Sahoo, U., Tiwary, A., RanjanSinha, V. (2021). Facile validated HPLC method using photodiode array detector for the combined analysis of etodolac and 5-FU in bulk and tablet dosage form. test, 64(3), 1601-1614. doi: 10.21608/ejchem.2020.45336.2922
Honey Goel; Richu Singla; Rakesh Chawla; Ujjwal Sahoo; Ashok Kumar Tiwary; Vivek RanjanSinha. "Facile validated HPLC method using photodiode array detector for the combined analysis of etodolac and 5-FU in bulk and tablet dosage form". test, 64, 3, 2021, 1601-1614. doi: 10.21608/ejchem.2020.45336.2922
Goel, H., Singla, R., Chawla, R., Sahoo, U., Tiwary, A., RanjanSinha, V. (2021). 'Facile validated HPLC method using photodiode array detector for the combined analysis of etodolac and 5-FU in bulk and tablet dosage form', test, 64(3), pp. 1601-1614. doi: 10.21608/ejchem.2020.45336.2922
Goel, H., Singla, R., Chawla, R., Sahoo, U., Tiwary, A., RanjanSinha, V. Facile validated HPLC method using photodiode array detector for the combined analysis of etodolac and 5-FU in bulk and tablet dosage form. test, 2021; 64(3): 1601-1614. doi: 10.21608/ejchem.2020.45336.2922

Facile validated HPLC method using photodiode array detector for the combined analysis of etodolac and 5-FU in bulk and tablet dosage form

Egyptian Journal of Chemistry
Article 48, Volume 64, Issue 3, March 2021, Pages 1601-1614    PDF (647.59 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2020.45336.2922
Authors
Honey Goel* 1; Richu Singla2; Rakesh Chawla3; Ujjwal Sahoo4; Ashok Kumar Tiwary5; Vivek RanjanSinha6
1University Institute of Pharmaceutical Sciences & Research, Baba Farid University of Health Sciences, Faridkot, India
2Department of Microbiology, Viral Research Diagnostics Laboratory (VRDL), Guru Gobind Singh Medical College and Hospital, Baba Farid University of Health Sciences, Faridkot, India
3Department of Pharmaceutical Chemistry, University Institute of Pharmaceutical Sciences and Research, Baba Farid University of Health Sciences, Faridkot
4Dr. B C Roy College of PHARMACY and AHS, Durgapur-713206, West Bengal
5Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India
6UIPS, Panjab University, Chandigarh
Abstract
The aim of the investigation was to develop and validate a reversed-phase high-pressure liquid chromatography (RP-HPLC) method for the simultaneous estimation of etodolac and 5-FU in bulk and coated tablet dosage form. The method was validated in terms of linearity, precision, accuracy, and specificity, LOD, and LOQ according to ICH guidelines. The standard instrument parameters were optimized using C18 reverse phase Inertsil® ODS (250 × 4.6 mm, 5µ particle size) column with system equipped with photodiode array (PDA) detector. An optimized ratio of mobile phase in RP-HPLC under low pressure gradient mode comprising of 60:40 % v/v of acetonitrile and di-potassium hydrogen phosphate buffer (pH 6.4; 25 mM), provided sharp peaks (having adequate molar absorptivity at λmax of 280 nm) with short retention time of 4.3+/-0.2 and 11.5+/-0.3 min for 5-FU and etodolac, respectively. Results indicated the method was linear, precise, accurate, rugged and robust with RSD values <2%. The percentage recoveries of both the drugs were within the limits of 90-110% at various spiked concentrations. The proposed method was found to be highly effective and could be successfully employed for the quantification of 5-FU and ETD in bulk drug and tablet formulations for routine analysis.
Keywords
RP-HPLC; 5-FU; ETD; validation; tablets; photodiode array (PDA)
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