A., G., M., E., A., I. (2013). Synergistic effect of gamma radiation and entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae on the humoral immune enzyme response in cotton leaf worm Spodoptera littolaris (Boisd).. test, 6(3), 1-10. doi: 10.21608/eajbsa.2013.13221
Gabarty A.; El-Sonbaty S. M.; Ibrahim A. A.. "Synergistic effect of gamma radiation and entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae on the humoral immune enzyme response in cotton leaf worm Spodoptera littolaris (Boisd).". test, 6, 3, 2013, 1-10. doi: 10.21608/eajbsa.2013.13221
A., G., M., E., A., I. (2013). 'Synergistic effect of gamma radiation and entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae on the humoral immune enzyme response in cotton leaf worm Spodoptera littolaris (Boisd).', test, 6(3), pp. 1-10. doi: 10.21608/eajbsa.2013.13221
A., G., M., E., A., I. Synergistic effect of gamma radiation and entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae on the humoral immune enzyme response in cotton leaf worm Spodoptera littolaris (Boisd).. test, 2013; 6(3): 1-10. doi: 10.21608/eajbsa.2013.13221

Synergistic effect of gamma radiation and entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae on the humoral immune enzyme response in cotton leaf worm Spodoptera littolaris (Boisd).

Egyptian Academic Journal of Biological Sciences. A, Entomology
Article 1, Volume 6, Issue 3, December 2013, Pages 1-10    PDF (214.77 K)
Document Type: Original Article
DOI: 10.21608/eajbsa.2013.13221
Authors
Gabarty A.1; El-Sonbaty S. M.2; Ibrahim A. A.3
1Department of Natural Products Research, National Center For Radiation Research and Technology, Atomic Energy Authority, Cairo, Egyp
2Department of Microbiology Research, National Center For Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt;
3Protection Research Institute, Agriculture Research Center, Egypt
Abstract
The present study was carried out to investigate the efficancy of substerilizing doses of gamma irradiation or/ and entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae on the humoral immune enzyme response of Spodoptera littolaris larvae. It was found that gamma irradiation of male pupae (50,100&150 Gy) decreases the humoral immune enzyme activity (phenoloxidase, prophenoloxidase, lysozyme) and protein concentration compared to the control of the 6th instar larvae S. littolaris. Injecting of  Lc50 concentration of both B. bassiana (0.4x105sp/µl)  and M. anisopliae (8x108sp/µl) to the last 5th instar larvae of S. littolaris were significantly increased phenoloxidase, prophenoloxidase, lysozym activities  and protein concentration after one day, however, they were significantly declined on the second, third and fourth days and remained at a level lower than that of the controls. Both gamma irradiation and entomopathogenic fungi (B. bassiana and M.anisopliae) significantly decreased the immune enzyme activity and protein concentration (p≤ 0.05) after 2, 3 & 4 days. However, this decrease was remarkable in the larvae of irradiated males’pupae (100Gy) which were injected with entomopathogenic fungi. In conclusion, gamma radiation and tested entomopathogenic fungi synergistically inhibitthe immune system of Spodopteralittolaris larvae.
Keywords
gamma radiation; Entomopathogenic fungi; Immune system
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