Sayed, A., Farrag, M., Abdelaty, B., Toutou, M., Muhammad, O. (2018). Histological alterations in some organs of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) produced using methyltestosterone. test, 22(4), 141-151. doi: 10.21608/ejabf.2018.13272
Alaa El-Din Sayed; Mahmoud Farrag; Basem Abdelaty; Mohammed Toutou; Ola Muhammad. "Histological alterations in some organs of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) produced using methyltestosterone". test, 22, 4, 2018, 141-151. doi: 10.21608/ejabf.2018.13272
Sayed, A., Farrag, M., Abdelaty, B., Toutou, M., Muhammad, O. (2018). 'Histological alterations in some organs of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) produced using methyltestosterone', test, 22(4), pp. 141-151. doi: 10.21608/ejabf.2018.13272
Sayed, A., Farrag, M., Abdelaty, B., Toutou, M., Muhammad, O. Histological alterations in some organs of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) produced using methyltestosterone. test, 2018; 22(4): 141-151. doi: 10.21608/ejabf.2018.13272

Histological alterations in some organs of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) produced using methyltestosterone

Egyptian Journal of Aquatic Biology and Fisheries
Article 10, Volume 22, Issue 4, September 2018, Pages 141-151    PDF (1.18 M)
Document Type: Original Article
DOI: 10.21608/ejabf.2018.13272
Authors
Alaa El-Din Sayed1; Mahmoud Farrag2; Basem Abdelaty3; Mohammed Toutou3; Ola Muhammad1
1Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt
2Faculty of science, Al-Azhar University (Assiut), Egypt
3National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Abstract
17α-methyltestosterone is the most efficient and least expensive method in monosex production in Egypt. This study was carried out to investigate the effect of this hormone on farmed Nile tilapia (Oreochromis niloticus) using some histological parameters. Fish samples were collected from four localities (Assiut; as a control and Beheira, Alexandria, Kafr El-Sheikh; as monosex populations). Histological sections in liver, kidney, and testis were prepared, stained, and examined according to histology protocol. Microscopic examination has been done for random samples showing some alterations, such as damages central vein (CV), nicrotic area (N), and hyperplasia in the hepatocytes (H) and Kuffer cells (Kc) in fish collected from Kafr El-Shiek farms. Sections of liver of tilapia from Alexandria showed hydropic degeneration (HD) in the hepatocytes (H), hyperplasia in the Kupffer cells (Kc) and blood congestion (BC) in the central vein.  Moreover, sections of liver of tilapia from Beheira showed the hepatocytes (H) and the central vein (CV) with presence of melanomacrophages (M) and hyperplasia in the hepatocytes and Kupffer cells (Kc).  Kidney of tilapia from monosex farms showed enlarged glomeruli (EG) and damaged renal tubules, detached epithelial cells and damaged renal tubules. Testis showed degeneration of the interstitial tissues and disorganization in the seminiferous tubules. It has been concluded that, the overdose of methyltestosterone (MT) induction affected some oranges in monosex fish.
Keywords
Oreochromis niloticus; monosex; methyltestosterone; melanomacrophages; Hyperplasia
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