Hussiny, S., Elissawy, A., Eldahshan, O., Elshanawany, M., Singab, A. (2020). Phytochemical investigation using GC/MS analysis and evaluation of antimicrobial and cytotoxic activities of the lipoidal matter of leaves of Sophora secundiflora and Sophora tomentosa. test, 4(2), 207-214. doi: 10.21608/aps.2020.38371.1039
Shaza Hussiny; Ahmed Elissawy; Omayma Eldahshan; Mohamed Elshanawany; Abdel-Nasser Singab. "Phytochemical investigation using GC/MS analysis and evaluation of antimicrobial and cytotoxic activities of the lipoidal matter of leaves of Sophora secundiflora and Sophora tomentosa". test, 4, 2, 2020, 207-214. doi: 10.21608/aps.2020.38371.1039
Hussiny, S., Elissawy, A., Eldahshan, O., Elshanawany, M., Singab, A. (2020). 'Phytochemical investigation using GC/MS analysis and evaluation of antimicrobial and cytotoxic activities of the lipoidal matter of leaves of Sophora secundiflora and Sophora tomentosa', test, 4(2), pp. 207-214. doi: 10.21608/aps.2020.38371.1039
Hussiny, S., Elissawy, A., Eldahshan, O., Elshanawany, M., Singab, A. Phytochemical investigation using GC/MS analysis and evaluation of antimicrobial and cytotoxic activities of the lipoidal matter of leaves of Sophora secundiflora and Sophora tomentosa. test, 2020; 4(2): 207-214. doi: 10.21608/aps.2020.38371.1039

Phytochemical investigation using GC/MS analysis and evaluation of antimicrobial and cytotoxic activities of the lipoidal matter of leaves of Sophora secundiflora and Sophora tomentosa

Archives of Pharmaceutical Sciences Ain Shams University
Article 3, Volume 4, Issue 2, December 2020, Pages 207-214    PDF (907.36 K)
Document Type: Original Article
DOI: 10.21608/aps.2020.38371.1039
Authors
Shaza Hussiny1; Ahmed Elissawy2; Omayma Eldahshan* 3; Mohamed Elshanawany4; Abdel-Nasser Singab5
1Department of Pharmacognosy, Faculty of Pharmacy, Badr University in Cairo, 11829, Cairo, Egypt.
2Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo POX 11566, Egypt
3Pharmacognosy Department, Faculty of Pharmacy, Ain Shams University, POX 11566, Cairo, Egypt.
4Pharmacognosy Department, Faculty of pharmacy, Badr University in Cairo (BUC), Egypt
5Department Pharmacognosy, Ain Shams University Vice President for Postgraduates studies and Research, Cairo, POX 11566, Egypt
Abstract
This study aims at investigation of the phytochemical composition, antimicrobial and cytotoxic activities of the lipoidal matter of leaves of S. secundiflora (Ortega) and S. tomentosa L.. The saponifiable and unsaponifiable matter of S. secundiflora and S. tomentosa leaves were assessed using GC/MS analysis. Where, saponification of lipoidal matter of S. secundiflora and S. tomentosa leaves yielded 31.55%, 87.74% for unsaponifiable matter and 19.66%, 38.70% for fatty acids methyl esters of both species, respectively. The dominant compounds in the unsaponifiable matter of S. secundiflora were β-amyrin acetate 55.20% and α-amyrin 9.73%. Whereas n-nonacosane 43.80% and 2-methyltriacontane 11.94% were the main components in S. tomentosa. In the saponifiable fraction, the content of saturated fatty acids identified in S. tomentosa 58.37% is higher than S. secundiflora 29.0%, while the percentage of unsaturated fatty acids identified in S. secundiflora 62.67% is higher than S. tomentosa 34.51%. Methyl linolenate 36.62% and methyl palmitate 40.02% are the major compounds in S. secundiflora and S. tomentosa, respectively. The lipoidal matters were evaluated in vitro for cytotoxic activity towards HCT-116 carcinoma cell line using the MTT assay with IC50 value of 97.00 and 38.76 μg/mL for S. secundiflora and S. tomentosa, respectively. Using the technique of agar well diffusion, the lipoidal matter of S. secundiflora and S. tomentosa displayed moderate antimicrobial activity at conc. of 50 mg/mL.
Keywords
Sophora secundiflora; Sophora tomentosa; cytotoxicity; anti-microbial; fatty acid methyl esters; GC/MS
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