Comparative Study between Croton tiglium Seeds and Moringa oleifera Leaves Extracts, after Incorporating Silver Nanoparticles, on Murine Brains | ||
| Egyptian Journal of Chemistry | ||
| Article 6, Volume 64, Issue 4, April 2021, Pages 1709-1731 PDF (1.32 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2021.53777.3113 | ||
| Authors | ||
| Wael M. Aboulthana* 1; Ahmed M. Youssef2; Mohamed M. Seif3; Noha M. Osman4; Ram Kumar Sahu5; Mohamed Ismael6; Hatim A. El-Baz1; Nagwa I. Omar7 | ||
| 1Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618). | ||
| 2Packaging Materials Department, National Research Centre, 33 Bohouth St., P.O. 12622, Dokki, Giza, Egypt (Affiliation ID: 60014618). | ||
| 3Toxicology and Food Contaminants Department, Food Industry and Nutrition Division, National Research Center | ||
| 4Cell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 Bohouth St., P.O. 12622, Dokki, Giza, Egypt (Affiliation ID: 60014618). | ||
| 5University College of Pharmacy, Pt. Deendayal Upadhyay Memorial Health Sciences and Ayush University of Chhattisgarh, Raipur (C.G.), India. | ||
| 6Energy and Environmental Sciences Laboratory, Chemistry Department, Faculty of Science, Sohag University, Egypt. | ||
| 7Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt affiliation ID: 60014618. | ||
| Abstract | ||
| Croton tiglium seeds and Moringa oleifera leaves extracts are rich in phytoconstituents with the antioxidant efficiency which can be enhanced by incorporating silver nanoparticles (Ag-NPs). The present study was designed to compare the effect of C. tiglium seeds and M. oleifera leaves nano-extracts on brain tissues of murine models. During the current study, acetylcholine esterase (ACHE), β-amyloid (Aβ) content and inflammatory markers were measured in brain tissues. Moreover, native protein, lipoprotein and isoenzymes patterns were electrophoretically detected. Also, the interferon-gamma (INF-γ) receptor protein was studied by molecular dynamic simulation to evaluate the significant alterations on brain tissues. It was found that ACHE, Aβ contents and inflammatory markers increased in the C. tiglium nano-extract treated group at a dose of 6.5 ml/kg. Furthermore, it caused qualitative electrophoretic abnormalities represented by lowering similarity index (SI) values. Also, the resides range 119~127 represent the most reactive and flexible site in INF-γ receptor protein. On the other hand, it was shown that no significant differences were induced by silver M. oleifera nano-extract. | ||
| Keywords | ||
| Croton tiglium Seeds; Moringa oleifera Leaves; Nanotechnology; Electrophoresis; Molecular Dynamic Modeling | ||
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