Etman, A., Abostate, H., El-Damasy, D., Soliman, D., Radwan, S. (2021). In silico and Microbiological studies for reduction of Pseudomonas aeruginosa virulence factors controlled by quorum sensing.. test, 1(1), 40-53. doi: 10.21608/aijpms.2021.52437.1008
Amira Etman; Heba Abostate; Dalia El-Damasy; Dalia Soliman; sahar Radwan. "In silico and Microbiological studies for reduction of Pseudomonas aeruginosa virulence factors controlled by quorum sensing.". test, 1, 1, 2021, 40-53. doi: 10.21608/aijpms.2021.52437.1008
Etman, A., Abostate, H., El-Damasy, D., Soliman, D., Radwan, S. (2021). 'In silico and Microbiological studies for reduction of Pseudomonas aeruginosa virulence factors controlled by quorum sensing.', test, 1(1), pp. 40-53. doi: 10.21608/aijpms.2021.52437.1008
Etman, A., Abostate, H., El-Damasy, D., Soliman, D., Radwan, S. In silico and Microbiological studies for reduction of Pseudomonas aeruginosa virulence factors controlled by quorum sensing.. test, 2021; 1(1): 40-53. doi: 10.21608/aijpms.2021.52437.1008

In silico and Microbiological studies for reduction of Pseudomonas aeruginosa virulence factors controlled by quorum sensing.

Azhar International Journal of Pharmaceutical and Medical Sciences
Article 5, Volume 1, Issue 1, January 2021, Pages 40-53    PDF (1.12 M)
Document Type: Original research articles
DOI: 10.21608/aijpms.2021.52437.1008
Authors
Amira Etman* 1; Heba Abostate1; Dalia El-Damasy1; Dalia Soliman2; sahar Radwan3
1Microbiology & Immunology department, Faculty of pharmacy, Egyptian Russian University
2Pharmaceutical Chemistry Department, Faculty of Pharmacy (Girls), Al-Azhar University.
3Microbiology and Immunology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt
Abstract
Aim: Inhibition of some quorum sensing (QS) regulated virulence factors via using non-antibiotic helping drugs to overcome antibiotic resistance problem. Introduction: P. aeruginosa is one of the major causes of fatal health care-associated infection. P. aeruginosa’s virulence factors are highly controlled by QS systems which lead to antibiotics resistance. Targeting QS may be a promising therapeutic hope for P. aeruginosa infections. Methods: Molecular Docking was performed by Shroedinger 2016 using the required constraints in the binding sites of the Las & Rhl receptor proteins of P. aeruginosa to estimate the expected activity of (quercetin and meloxicam) and compare them with great docking scores compounds like rosmarinic acid. PAO1 was used as reference strain with other P. aeruginosa strains (clinical samples). An antibiotic sensitivity test was performed to evaluate antibiotic resistance. The minimum inhibitory concentration (MIC) of tested drugs were also measured against the resistant strains. Virulence factors thanked for QS (protease, pyocyanine, rhamnolipids) and biofilm formation of selected strains were assessed before and after treatment with quercetin & meloxicam. Results and conclusion: In silico studies indicated that quercetin and meloxicam could act as inhibitors for the auto-inducer molecules as they have a high affinity for the regulatory proteins of the QS system lasR and rhlR. The experimental study of quercetin and meloxicam against P. aeruginosa showed that at sub-MIC, both drugs reduced biofilm formation and some tested virulence factors. Treatment of P. aeruginosa infection using QS inhibitors is expected and this approach may overcome the antibiotic resistance problem.
Keywords
Quorum sensing; P. aeruginosa; Virulence factors; Quercetin; Meloxicam
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