Efficiency of Citrus limon extract on biological and molecular activities of Biomphalaria alexandrina snails | ||
| Delta Journal of Science | ||
| Article 8, Volume 36, Issue 3, December 2013, Pages 186-200 PDF (2.55 M) | ||
| Document Type: Research and Reference | ||
| DOI: 10.21608/djs.2013.139774 | ||
| Authors | ||
| Sherin K. Sheir; Azza H. Mohamed; Gamalat Y. Osman; Sobhy E. Hassab EL-Nabi; Shaimaa A. Allam | ||
| Department of Zoology, Menoufia University, Shebeen El-koom, Egypt. | ||
| Abstract | ||
| Molluscicides have gained a great attention in recent years and proved to be a good way of snail control. C. limon was tested as a molluscicide against adult Biomophalaria alexandrina snails and a larvicide against Schistosoma mansoni larvae (miracidia and cercariae). Lethal concentrations, LC50 and LC90 values were detected for both tests (744.17 and 1821.25 ppm, respectively). B. alexandrina snails, non-infected and infected with S. mansoni were exposed to Citrus limon peel extract concentration of 150 ppm for 4 weeks compared to non expoed with C. limon and/or infected controls. The meracidea, cercariae, egg production, protein electrophoresis and DNA fragmentation analysis and organ histology of B. alexandrina were assessed. C. limon exposure stimulated the growth rate, increased the number of eggs/snail/week (59.31 ± 5.71), inhibited reproductive rate by 20.6 %, hatchability rate (57.7 %) and stimulation of cercarial production. Histological investigation of C. limon extract exhibited necrosis and deformation of hermaphrodite and digestive glands of infected and non-infected B. alexandrina snails. C. limon exposure led to increase in total protein intensity of the hermaphrodite-digestive glands in both exposed/exposed-infected snails. RNA intensity was increased in C. limon exposed and exposed-infected snails during the experimental period. Necrotic DNA form was a characteristic of C. limon and/or infection. | ||
| Keywords | ||
| Citrus limon; Biomphalaria alexandrina; molluscicide; proteins; DNA damage | ||
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