Genetic Differentiation of Citrullus colocynthis (L.) Populations Depending on Allozyme Diversity | ||
| Egyptian Journal of Botany | ||
| Article 22, Volume 58, Issue 3, December 2018, Pages 581-590 PDF (776.12 K) | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2018.3595.1170 | ||
| Authors | ||
| Shawkat M. Ahmed* 1, 2; Khalid H. Alamer3 | ||
| 1Biology Department, Faculty of Science, Taif University, PO Box 5700,Taif, KSA | ||
| 2Biology Department, Faculty of Education, Ain Shams University, Cairo, Egypt | ||
| 3Biology Department, Faculty of Science, Taif University, PO Box 5700, Taif, KSA | ||
| Abstract | ||
| CITRULLUS colocynthis (L.) is a desert plant having a large history in pharmaceutical industries as an important medicinal plant. Allozyme variation and population genetic structure of C. colocynthis in 6 natural populations from western region, Saudi Arabia, were investigated by 6 enzyme systems. The genetic differentiation within and among C. colocynthis populations has been revealed by allozyme analysis. Ten loci were monomorphic in all populations, whereas seven loci having two alleles (α-EST-1, β-EST-1, MDH-1, ADH-4, ADH-5, ALO-4 and PRX-2) were polymorphic. The mean allozyme variability indices within C. colocynthis populations (P = 31.17%; A = 1.21) were lower than those for insect-pollinated species (P = 34%; A = 2.67; He = 0.205) except He value (0.415) was higher. The observed mean heterozygosity (Ho= 0.757) was higher than the expected mean heterozygosity (He= 0.415) indicating the existence of natural selection against homozygosity. The Inbreeding coefficient values (F) of polymorphic loci were negative suggesting a significant excess of heterozygosity in the studied populations. The UPGMA dendrogram of 84 individuals of C. colocynthis confirmed the notable variability within populations. Differentiation among-population (FST) recorded 16.2% of the total variation. Low FST and high genetic distance (D) values suggested a low differentiation among C. colocynthis populations. Little gene flow (Nm ) was deduced in all populations except ME based on the allozyme data. We suggest that population ME can be recommended for both genetic conservation and breeding programs. | ||
| Keywords | ||
| <i> Citrullus; Allozyme </i>; population; heterozygosity; Gene flow | ||
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