Mahmoud, H., Khater, D., Abd El Wahed, S. (2021). Evaluation of Anti-cancer Effect of Different Concentrations of Black Raspberries Extract on Oral Squamous Cell Carcinoma Cell Line (In Vitro Study). test, 67(Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), 419-429. doi: 10.21608/edj.2020.50388.1349
Hatem Mahmoud; Dina Khater; Sara Abd El Wahed. "Evaluation of Anti-cancer Effect of Different Concentrations of Black Raspberries Extract on Oral Squamous Cell Carcinoma Cell Line (In Vitro Study)". test, 67, Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), 2021, 419-429. doi: 10.21608/edj.2020.50388.1349
Mahmoud, H., Khater, D., Abd El Wahed, S. (2021). 'Evaluation of Anti-cancer Effect of Different Concentrations of Black Raspberries Extract on Oral Squamous Cell Carcinoma Cell Line (In Vitro Study)', test, 67(Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), pp. 419-429. doi: 10.21608/edj.2020.50388.1349
Mahmoud, H., Khater, D., Abd El Wahed, S. Evaluation of Anti-cancer Effect of Different Concentrations of Black Raspberries Extract on Oral Squamous Cell Carcinoma Cell Line (In Vitro Study). test, 2021; 67(Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)): 419-429. doi: 10.21608/edj.2020.50388.1349

Evaluation of Anti-cancer Effect of Different Concentrations of Black Raspberries Extract on Oral Squamous Cell Carcinoma Cell Line (In Vitro Study)

Egyptian Dental Journal
Article 12, Volume 67, Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology) - Serial Number 3, January 2021, Pages 419-429    PDF (927.57 K)
Document Type: Original Article
DOI: 10.21608/edj.2020.50388.1349
Authors
Hatem Mahmoud1; Dina Khater2; Sara Abd El Wahed* 3
1B.D.S (Cairo University) and Demonstrator of Oral and Maxillofacial Pathology, Faculty of Dentistry, The British University in Egypt.
2Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Cairo University.
3Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, The British University in Egypt.
Abstract
Background: Black Raspberry has a wide range of pharmacological and therapeutic aspects including anti-inflammatory and antioxidant activities. Moreover, the effect of Black Raspberry has been studied in many cancers such as human cervical, esophageal and colorectal cancer and showed cytotoxic and anti-proliferative effects against these types of cancer.
Aim: To identify the possible anti-cancer effect of Black Raspberry on Oral Squamous Cell Carcinoma cell line.
Material and methods: Human tongue squamous cell carcinoma cell line was divided into 3 groups: one control group, second group treated with Black Raspberry, and the third group treated with Doxorubicin, each of the two treatments was applied for 24, 48, 72 hours, respectively. Then, cellular viability was measured using Microculture Tetrazolium assay, cell cycle analysis was done using Flow Cytometry and eventually, apoptotic activity was evaluated using Enzyme-linked Immunosorbent assay.
Results: Black Raspberry succeeded to decrease the percentage of viable and proliferating cells with increasing time. On the other hand, it increased the percentage of apoptotic cells and levels of caspase-3.
Conclusion: Black Raspberry has a potential cytotoxic effect on oral squamous cell carcinoma cell line in a dose and time dependent manner, and exerts this action through induction of apoptosis, and its action is comparable to Doxorubicin action.
Keywords
Black Raspberry; Doxorubicin; Oral Squamous Cell Carcinoma
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