Hassan, M., Tartor, Y., Ashour, A., Elmowalid, E. (2018). Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans. test, 46(2), 128-135. doi: 10.21608/zvjz.2018.14385
Mohamed N. Hassan; Yasmine H. Tartor; Ashour F. Ashour; Elmowalid A. Elmowalid. "Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans". test, 46, 2, 2018, 128-135. doi: 10.21608/zvjz.2018.14385
Hassan, M., Tartor, Y., Ashour, A., Elmowalid, E. (2018). 'Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans', test, 46(2), pp. 128-135. doi: 10.21608/zvjz.2018.14385
Hassan, M., Tartor, Y., Ashour, A., Elmowalid, E. Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans. test, 2018; 46(2): 128-135. doi: 10.21608/zvjz.2018.14385

Enterococcus faecalis Cell-Free Supernatant Inhibits Hyphal Morphogenesis and Biofilm Formation in Candida albicans

Zagazig Veterinary Journal
Article 5, Volume 46, Issue 2, June 2018, Pages 128-135    PDF (622.81 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2018.14385
Authors
Mohamed N. Hassan1; Yasmine H. Tartor* 1; Ashour F. Ashour2; Elmowalid A. Elmowalid1
1Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
2Public Health Department, Dakahlia Veterinary Medicine Directorate, El Mansoura, Egypt
Abstract
Enterococcus faecalis and Candida albicans are two of the most significant opportunistic and intricate nosocomial pathogens. They reside overlapping niches as major constituents of the gastrointestinal (GI) and oral microbiome. This study was designed to investigate the interaction between these two opportunistic pathogens that could affect treatment strategies and influence the interkingdom signaling and sensing in the microbiome conceptions. E. faecalis was isolated from 150 samples collected from human urine, cattle raw milk, milk products and chicken intestine and identified by both the routine microbiological and molecular identification methods based on 16S rRNA gene. Isolates susceptibility to nine antimicrobials was analyzed using the disk-diffusion method and E. faecalis isolates showed multidrug resistance. Most of C. albicans isolates were able to form biofilm with different intensities. Cell-free supernatant (CFS) of E. faecalis was purified and tested against C. albicans and/or biofilm formation.It inhibited the growth of C. albicans and their filamentation with regards to biofilm formation in vitro. The data presented here demonstrated the potential antifungal activity of E. faecalis CFS and could explain the biological interaction between these two microbes in one hand and the possibility of using E. faecalis CFS as a biological product to control yeast infection in the other hand.
Keywords
C. albicans; E. faecalis; 16S rRNA; Biofilm; Anti-Candida factor
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