El-Kholy, M., W.T., K., A.S., M. (2011). TAXONOMIC EVALUATION USING POLLEN GRAIN SCULPTURE AND SEED COAT CHARACTERS OF 11 TAXA OF GENUS HIBISCUS (MALVACEAE) IN EGYPT. test, 19(1), 143-152. doi: 10.21608/ajs.2011.14637
M.A El-Kholy; Kasem W.T.; Mabrouk A.S.. "TAXONOMIC EVALUATION USING POLLEN GRAIN SCULPTURE AND SEED COAT CHARACTERS OF 11 TAXA OF GENUS HIBISCUS (MALVACEAE) IN EGYPT". test, 19, 1, 2011, 143-152. doi: 10.21608/ajs.2011.14637
El-Kholy, M., W.T., K., A.S., M. (2011). 'TAXONOMIC EVALUATION USING POLLEN GRAIN SCULPTURE AND SEED COAT CHARACTERS OF 11 TAXA OF GENUS HIBISCUS (MALVACEAE) IN EGYPT', test, 19(1), pp. 143-152. doi: 10.21608/ajs.2011.14637
El-Kholy, M., W.T., K., A.S., M. TAXONOMIC EVALUATION USING POLLEN GRAIN SCULPTURE AND SEED COAT CHARACTERS OF 11 TAXA OF GENUS HIBISCUS (MALVACEAE) IN EGYPT. test, 2011; 19(1): 143-152. doi: 10.21608/ajs.2011.14637

TAXONOMIC EVALUATION USING POLLEN GRAIN SCULPTURE AND SEED COAT CHARACTERS OF 11 TAXA OF GENUS HIBISCUS (MALVACEAE) IN EGYPT

Arab Universities Journal of Agricultural Sciences
Article 9, Volume 19, Issue 1, March 2011, Pages 143-152    PDF (914.11 K)
Document Type: Original Article
DOI: 10.21608/ajs.2011.14637
Authors
M.A El-Kholy* ; Kasem W.T.; Mabrouk A.S.
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Egypt
Abstract
Pollen grain morphology and seed coat characters of 11 cultivars belonging to two species of genus Hibiscus (Family Malvaceae) namely H. esculentus (H. Abelmoschus) and H. sabdariffa were investigated. This study was carried out using light microscope (LM) and Scanning Electron Microscopy (SEM). Pollen morphology of this genus is fairly uniform. Generally radially symmetrical apolar, mostly spheroidal, pantoporate. Seed exomorphic characters revealed four types of ornamentations; reticulate, ocealate,foveolate andruminate. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was employed to characterize those taxa. Thirty one bands of seed protein profiles have been constructed from the gel. The produced dendrograms were analyzed by STATISCA program using UPGMA clustering method showed a close affinity among the seven H. esculentus cultivars and the four H. sabdariffa cultivars.
 
Keywords
Pollen grains morphology; Hibiscus; seed coat; SDS-PAGE
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