BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SOME YEAST ISOLATES | ||
| Arab Universities Journal of Agricultural Sciences | ||
| Article 8, Volume 15, Issue 2, September 2007, Pages 315-324 PDF (306.51 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajs.2007.14843 | ||
| Authors | ||
| A. Abulhamd* 1; M M Mokhtar* 2; Rasha Mohamed Farrag,2 | ||
| 1Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt | ||
| 2The Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt | ||
| Abstract | ||
| Sixteen yeast isolates from various sources in-cluding molasses, foodstuffs, concentrated juices and soils were identified using simplified API 20 C AUX yeast identification system and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The isolates were found to belong to 11 species; Candida famata, C. glabra-ta, C. krusei / inconspicua, C. norvegensis, C. col-liculosa, Cryptococcus humicolus, Rhodotorula mucilaginosa, Kloeckera spp., Geotrichum capita-tum and Saccharomyces cerevisiae. API and RAPD-PCR proved to be useful and convenient taxonomic tools for rapid identification at the spe-cies level, proving that at least two techniques are needed for precise identification. RAPD-PCR pro-cedure is faster than traditional characterization techniques; the identification of a single colony was typically achieved in less than 10 hr. | ||
|
Supplementary Files
|
||
|
Statistics Article View: 195 PDF Download: 357 |
||
