Heba, O., Wedad, E.E., E., Sawsan, F., S., H.H., A. (2005). ISOLATION, PURIFICATION AND IDENTIFICATION OF SOME MICROORGANISMS PRODUCE PLANT GROWTH PROMOTING SUBSTANCES (METHYLOTROPHIC BACTERIA). test, 13(3), 717-729. doi: 10.21608/ajs.2005.15280
Orf, O.M. Heba; Eweda Wedad, E.E.; Shehata Sawsan, F.; Abo-Taleb H.H.. "ISOLATION, PURIFICATION AND IDENTIFICATION OF SOME MICROORGANISMS PRODUCE PLANT GROWTH PROMOTING SUBSTANCES (METHYLOTROPHIC BACTERIA)". test, 13, 3, 2005, 717-729. doi: 10.21608/ajs.2005.15280
Heba, O., Wedad, E.E., E., Sawsan, F., S., H.H., A. (2005). 'ISOLATION, PURIFICATION AND IDENTIFICATION OF SOME MICROORGANISMS PRODUCE PLANT GROWTH PROMOTING SUBSTANCES (METHYLOTROPHIC BACTERIA)', test, 13(3), pp. 717-729. doi: 10.21608/ajs.2005.15280
Heba, O., Wedad, E.E., E., Sawsan, F., S., H.H., A. ISOLATION, PURIFICATION AND IDENTIFICATION OF SOME MICROORGANISMS PRODUCE PLANT GROWTH PROMOTING SUBSTANCES (METHYLOTROPHIC BACTERIA). test, 2005; 13(3): 717-729. doi: 10.21608/ajs.2005.15280

ISOLATION, PURIFICATION AND IDENTIFICATION OF SOME MICROORGANISMS PRODUCE PLANT GROWTH PROMOTING SUBSTANCES (METHYLOTROPHIC BACTERIA)

Arab Universities Journal of Agricultural Sciences
Article 10, Volume 13, Issue 3, September 2005, Pages 717-729    PDF (1.4 M)
Document Type: Original Article
DOI: 10.21608/ajs.2005.15280
Authors
Orf, O.M. Heba* 1; Eweda Wedad, E.E.2; Shehata Sawsan, F.3; Abo-Taleb H.H.3
1Department of Agricultural Microbiology, Soils, Water & Environment Research Institute, Agricultural Research Center, Giza, Egypt.
2Department of Agricultural Microbiology, Soils, Water & Environment Research Institute, Agricultural Research Center, Giza, Egypt
3Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams Uni-versity, Shoubra El-Kheima, Cairo, Egypt.
Abstract
Recently, the potential economical importance of the methylotrophic bacteria encouraged the isolation of this group. In the present study five Egyptian isolates were obtained from green leaves surface of legume plants named PPFM.C (ChickPea), PPFM.Ph (Common bean), PPFM.F (Faba bean), PPFM.P (Peanut) and PPFM.S (Soybean) ,to study their general characters which belonging to  methylotrophic bacteria. Morphological studies indicate that all isolates were short rods, gram negative and motile. All Physiological studies to the isolates gave the same results except PPFM.F which could not grow in peptone medium. All isolates were sensitive to Kanamycin but they were resistant to Erythromycin. There was a great range in the ability of the isolates to grow on different sodium chloride concentrations indicating that PPFM.Ph grew well in 5 % sodium chloride, and they were able to excrete and produce cytokinin. Molecular biology studies indicated that there was a great similarity between PPFM.C and PPFM.Ph (99.34%). Identification was carried out to the5 isolates, PPFM.F may be related to Methylobacterium mesophilicum, PPFM.P may be related to M. fujisawaense and PPFM.Ph, PPFM.C and PPFM.S were related to M. radiotolerans. 
Keywords
Methylotrophs; Pink-Pigmented Facultatively Methylotrophic (PPFM); Green leaves of legume plants; Cytokinin; Methylobacterium sp
Supplementary Files
Statistics
Article View: 225
PDF Download: 298