PREPARATION OF MONOCLONAL ANTIBODIES AGAINST INFECTIOUS LARYNGOTRACHEITIS VIRUS (ILT) | ||
| Egyptian Journal of Agricultural Research | ||
| Article 14, Volume 93, Issue 1, March 2015, Pages 197-209 PDF (3.6 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2015.153328 | ||
| Authors | ||
| MOHAMED M., YOUSSEF; HANAN M. ELZAHED; AMAL EL SAWAH; ELHAM A. ELEBIARY | ||
| Central Laboratory For Evaluation Of Veterinary Biologics (CLEVB), ARC, Dokki, Giza, Egypt | ||
| Abstract | ||
| hybridomas which secrete monoclonal antibodies (MAbs) against Infectious laryngotracheitis virus (ILTV) were produced. The hybrid cells resulted from fusion of P3X63- Ag8.653 myeloma cells with splenocytes of Balb/c mice which had been immunized with purified ILTV. After immunization the humoral immune response to immunizations was assessed by ELISA using purified ILTV as the target. Screening of supernatant fluids after cell fusion was performed by an indirect enzyme-linked immunosorbent assay (ELISA). The results indicated that the obtained (MAbs) against ILTV were of high specificity and with ambient concentration that would be useful as a tool for diagnosis and identification of ILT virus. | ||
| Keywords | ||
| MAbs / ILTV / antibody production/ Hybridoma; Technology/ ELISA) | ||
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