Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures | ||
| Journal of Applied Veterinary Sciences | ||
| Article 1, Volume 6, Issue 2, April 2021, Pages 1-14 PDF (687.08 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2021.154573 | ||
| Authors | ||
| Salwa S.Thabet1; Manal H. Thabet* 2 | ||
| 1Dept. of Food Hygiene, Animal Health Research Institute (Regional Laboratory, Assiut) , Agriculture Research Center (ARC), Egypt. | ||
| 2Dept. of Bacteriology, Animal Health Research Institute (Regional Laboratory, Assiut) , Agriculture Research Center (ARC), Egypt. | ||
| Abstract | ||
| Yersinia enterocolitica is considered one of the most prevalent pathogens transmitted through milk and milk products. Therefore, we aimed to detect the prevalence of these bacteria in cheese and ice cream and study the influence of refrigeration and freezing on its growth patterns. A total of 80 samples of cheese and ice cream were collected from Assiut city, Egypt (40 samples each). The collected samples were examined for the isolation of Y. enterocolitica by the classical culture method and improved by the PCR technique. The incidence of Y. enterocolitica was 17.5 and 25.0 % in the examined cheese and ice cream samples by culture method, respectively, its prevalence in the tested cheese and ice cream samples basing on PCR were 7.5 and 15% since 42.8 and 60.0% of the isolated Y. enterocolotica were confirmed positive. Bio- typing and serotyping of the isolated strains revealed that 8 out of the confirmed strains were pathogenic ; Y. enterocolitica serotype O: 3 was the most prevalent strain, and all of the pathogenic strains carried the virulent ail gene. Nearly similar growth patterns of Y. enterocolitica were recorded during storage of cheese at 4 ± 2°C and 30 ± 2ºC and, a significant difference was observed in the 3rd week, the organism found to be survived for 18 weeks with a mean value of 7.4 ± 0.5 and 6.9 ± 0.16 log cfu/ g for cheese stored at 4 ± 2°C and 30 ± 2°C respectively. In the case of ice cream, there was a significant difference between the behavior of Y. enterocolitica during freezing storage at -6 ± 2°Cand -18± 2ºC in the 2nd week, the mean value for the organism count on the 16th week was 5.3 ± 0.26 and 5.6 ± 0.39 log cfu/ g, respectively. | ||
| Keywords | ||
| Cheese; Ice cream; PCR; Virulence genes; Y. enterocolitica | ||
| References | ||
|
ABD EL- FATAH, EMAN, N., AMER, I. H., ELSAYED, M. S. AND MANSOUR, M. A. H. .2015. Assessment of the effect of freezing on the survival of some pathogenic bacteria in ice cream. J. Global. Biosci, 4(7) 2873-2877.
ABD EL-AAL, S. F. A. AND ATTA, M. A-H. B. .2009. Occurrence of listeria and Yersinia spp. in milk and some milk products. Assiut Vet. Med. J., 55 (123): 45- 59.
ABDOLLAH, ELHAM AND HANAFIAN, SHARMA .2015. Behavior of
Y. enterocolitica in UF white cheese: impact of different storage temperature on various strains .J. Food Safety, 36(2): 247- 253.
ABOU EL- MAKAREM, H. S. M. .2009. Plasmid Profile for several pathogenic microorganisms strains isolated from market kareish cheese. M. V. Sc. Thesis, Faculty of Vet. Med., Alexandria.
ALI, AMANY, M., ATTIAH, S. A. AND ALY, SALWA, A. .2015. Incidence of Y .enterocolitica in milk and some milk products. Inl. J. Adv. Biol. Res., 5(4): 285- 288.
ALI, MAY, M. AND AL-SAMARI, F. R. .2020. Identification and characterization of virulent Y. enterocolitica in locally produced soft cheese in Iraq. Plant Archives, 20 (2): 6595- 6600.
AMIN, M. AND DRAUGHON, F. A. .1987. Growth characteristic of Yersinia enterocolitica in Pasteurized skim milk, J. Food Port. , 50; 275-281.
ANNA, F. AND JORDI, V. .2012. Y. enterocolitica: Pathogenesis, virulence and antimicrobial resistance. Enfermeda des Ifecciosas y microbiologia, 30 (1): 24- 32.
BAHGAT, N. AND VIRDI, J. S. .2007. Distribution of virulence-associated genes in Y. enterocolitica biovar 1A correlates with clonal groups and not the source of isolation FEMS Microbiol., 266 (2): 177- 183.
BOLTON, D. J., IVORY, C. AND MACDOWELL, D. .2013. A small study on Y. enterocolitica in pigs from birth to carcasses and characterization of porcine and human strains. Food Control, 33: 521- 524.
BOTTONE, E. J. .1997. Y. enterocolitica: the charisma continues. Clin. Microbiol. Rev., 10: 257- 276.
CHAMPAGNE, PC, LAING R. R.; ROY D.; MAFU A. A.; GIFFITHS W. M. AND WHITE C. .1914. Psychrotrophs in dairy products: Their effects and their control. Food Sci.Nutr 34(1):1-30
CHENYANG L., MURUGAIYAN J., THOMAS C., ALTER T. AND RIEDEL C. .2020. Isolate Specific Cold Response of Yersinia enterocolitica in Transcriptional. Front. Microbiol. 10: 3037-3053.
DOYLE, M. E. .1990. Pathogenic E. coli, Y. enterocoliticaand Vibrio parahaemolyticus. Lancet 336, 1111.
EL-MALT, LAILA, M. .2009. Occurrence of Coliformis and Yersinia spp. in raw milk and kareish cheese in Quena Governorate, 37(3): 32.
EL- SHERBINI, M., AL-AGILI, S., EL-JALI, H., ABOSHKIWA, M. AND KOHA, M. .1999. Isolation of Y. enterocolitica from cases of acute appendicitis and ice cream. East Mediterr. Health J., 5(1): 130- 135.
FDA/ CFSAN/ BAM .2007. Y. enterocolitica Bacteriological Analytical Manual online chp.8. US Food and Drug Administration Center for Food Safety and Applied Nutrition. Weagant, S.D. and Feng, P., http://www.cfsan.fda.gov/~ebam/bam.8.html,2001,updat2007.
FREDRIKSSON- AHOMAA, M. AND KORKEALA .2003. Low occurrence of pathogenic Y. enterocolitica in clinical food environmental samples: a methodological problem. Clinical Microbid. Reviews, 16 (2): 220- 229.
FREDRIKSSON- AHOMDA, M., LINDRSTROM, M. AND KORKEATA, H. .2010. Y. enterocolitica and Y. pseudotuberculosis in Juneau, V. K. and Sofos, N. J. (Eds), Pathogen and Toxins in Foods Challenger and Intervention. ASM press. Washington, D. C., 164- 180.
GRAM, L., RAVN, L., RASCH, M., BRUH, J. B., CHRISTENSEN, A. B. AND GIVSKOV, M. .2002. Food spoilage interactions between food spoilage bacteria. Int. J. Food Microbiol., 78: 79- 97.
GOUNOT, A. M. .1991. Bacterial life at low temperatures: physiology aspects and biotechnological implication. J. Appl. Bacteriol., 71: 386- 397.
HAMAMA, A., EL-MARRAKCHI AND OTHMANI, F. .1992. Occurrence of Yersinia enterocolitica in milk and dairy milk products in Morocco. Inter. J. Food Microbiol., 16: 69-77
HANAFIAN, S. AND KHANI, S. .2012. Fate of Y. enterocolitica during manufacture, ripening and storage of Lighvan cheese. Int. J. Food Microbiol., 156:141-146.
HANAFIAN, S. AND KHANI, S. .2012. Prevalence of virulent Y. enterocolitica and Y. pseudoluberculosis in bulk raw milk and retail cheese in northern-west of Iran. Int. J. Food. Microbiol., 155: 89- 92.
HARAKEH, E. SALEH, I., BARBOUR, E. AND SHAIB, H. .2012. Highly resistant Y .enterocoliticaisolated from dairy-based foods in Lebanon. Int. Arabic J. Antimicrob. Agents, 2(1):
HARTING, M. AND GERIGK, K. .1991. Yersinia in effluents from food processing industry. Rev. Sci. Tech., 10: 799- 807.
ILIEV, M. AND NAJDENSKI, H. .2008. Monitoring of plasmid dissociation and pathogenic potential among Y. enterocolitica and Y. pseudotuberculosis during storage of refrigerated pork meet. Anna's of Microbiol., 58 (4): 623- 632.
JAY, J. M. .1996. Modern food microbiol. New York, Champan& Hall.
KHALIFA, NASHWA, O., ABOU ELROOS, NAHLA, A. AND SHARAF, EMAN, M. .2007. Public health importance of Y. enterocolitica in ice cream peddler in Kaliobia Governorate. Alex. J. Vet. Sci., 26 (1): 51- 54.
LANTZ, P. G., KNUTTSSONA, R., BLIXTB, Y., ABU AL-SOUDA, W. BORCH, E. AND RADOSTORM, P. .1998. Detection of pathogenic Y. enterocolitica in enrichment media and pork by a multiplex PCR: A study of sample preparation and PCR- inhibitory components. Int. J. Food Microbiol., 45: 93- 105.
MACFADDIN, J. F. .2000. Biochemical tests for identification medical bacteria. Warery Press Inc, Baltimore, Md. 21202 USA.
MANAFI, M., AND HOLZHAMMER, E. .1994. Comparison of the Vitek, API 20E and Gene trak® systems for the identification of Y. enterocolitica. Letters in Appl. Microbiol., 18: 90- 92.
MYERS, K. M., GABA, J. AND AL-KHALDI, S. F. .2006. Molecular identification of Y. enterocolitica isolated from pasteurized whole milk using DNA Microarray chip hybridization. Mol. Cell. Probes, 20: 71- 80.
NEUBAUER, H., SPRAGUE, L. D., SCHOLZ, H. AND HENSEL, A. .2001. Diagnosis of Y. enterocolitica infections: a review on classical identification techniques and new molecular biological methods. Berliner und Munchenertierarztliche Wochenschrift, 114 (1-2), 1-7
NORMA, B. P. AND ANA, M. S. .2000. Isolation of Y.enterocolitica in ice cream at different pH values Stored at -18± 2ºC. Brazilian J. Microbiol., 31: 174- 177.
NUSRAT, S., DEVANG, S. AND SUFIAN, F. .2009. Detection of Y. enterocolitica in alfalfa, Mung Bean, Cilantro and mameysapote (Pouteria sapota). Food Matrices using DNA Microarray chip hybridization. Curr. Microbiol., 59: 233- 239.
OTERO.V. L., ESTRAD, C. L., FAVIER, G. I., VELAZQUEZ, L., ESCUDERO, MARIA, E. AND SLEFANINI DE GUZMA, ANA MARIA.2010. Detection and survival of Y. enterocolitica in goat cheese produced in San Luis, Argentina. J. Food Safely, 30(4): 982- 995.
RAHIMI, E., SEPEHRI, SARA, DEHKODI, F. S., SHAYGAN, SHIMA AND MOMTAZ, H. .2014. Prevalence of Yersinia spp. in traditional and commercial dairy products is Isfahan Province, Iran. Jundishapur J. Microbiol., 7 (4): 249- 255.
RAKESH, K. .2007. Advances in Thermal and Non-Thermal Food Preservation (Aseptic Processing) (pp.43). 1st ed. Edited by Black Well Publishing Ltd, Oxford, UK.
ROORA, S., BUDU- AMOAKO, E., ABLETT, R. F.AND SMITH, J. .1992. Effect of high temp- short-time pasteurization, freezing and thawing and constant freezing on the survival of Y. enterocolitica in milk. J. Food Prot., 55: 803- 805.
SCHRIEFER, M. E. AND PETERSEN, J. M. .2011. Yersinia. In: Versalovic, J., Caroll, K. C., Funke, G., Jorgensen, J. H., Landry, M. L. and Warnock, D. W., Ed. Manual of Clinical Microbiology. 10th ed. Washington: American Society for Microbiology Press 627- 238.
SEVERGNINI, M., CREMONESI, P., CONSOLANDI, C., DE BELLIS, G. AND CASTIGLIONI, B. .2011. Advances in DNA microarray technology for the detection of food-borne pathogens. Food Bioprocess Tech., 4 : 936- 953.
SHURNIK, M. AND TOIVONEN, S. .2011. Identification of distinct lipopolysacharid patterns among Y.enterocolitica and Y.enterocolitica like bacteria. Biochemistry (MOSC), 76- (7): 823- 831.
SLAVCHEVE, G. .1986. Development and survival of Y. enterocolitica in pasteurized milk and ice cream Vet. Med. Nauki., 23(6):77-85.
STERN, N.J., PIERSON, M. D. , AND KOTULA, A. W. .1980. Growth and competitive nature of Yersinia enterocolitica in whole milk, J. Food Sci., 45; 64-70.
TENNAT, S.; GRANT, T. AND BROWNE, R. .2003. Pathogenicity of Y. enterocolitica biotype 1A. FEMS Immunol. Med. Microbiol., 38: 127-137.
THOERNER, P., BIN KINGOMBE, C. I., BOGLI- SLUBER, K., BISSIGCHOISAT, B., WASSENAAR, T. M., FREY, J. AND JEMMI, T. .2003. PCR detection of virulence genes in Y.
enterocolitica and Y. pseudoluberculosis and investigation of virulence gene distribution. Appl. Environ. Microil., 69: 1810- 1816.
UZUNLU, S., YILDIRIM, I. AND DEMIR, M. .2004. Survival characteristics of some pathogenic bacteria in vanilla ice cream at different storage periods. Turk. Mikobiyol. Cem. Derg., 34:195-199.
VAZLEROVA, M. AND STEINHAUSEROVA, I. .2006. The comparison of the methods for identification of pathogenic serotypes and biotypes of Y. enterocolitica: microbiological methods and PCR. Czech J. Food Sci., 24: 217- 222.
VISHNUBHATLA, A., OBERST, R. D., FUNG, D. Y. C., WONGLUMSOM, W., HAYS. M. P., AND NAGARAJA, T. G. .2001. Evaluation of a 52 -nuclease (TaqMan) assay for the detection of virulent strains of Y. enterocolitica in raw meat and tofu samples. J. Food Prot., 64: 355-360.
WANNET, W. J., REESSINK, M., BRUNINGS, H. A., MAAS, H. M. .2001. Detection of pathogenic Y. enterocolitica by a rapid and sensitive duplex PCR assay. J. Clin. Microbiol, 39(12):4483- 6.
WARKE, R., KAMAT, A., KAMAT, M. AND THOMAS, P. .2000. Incidence of pathogenic psychotrophes in ice cream sold in some retail outlets in Mumbai, India. Food Cont., 11: 77- 83.
YE, Y. W., LING, N., HAN, Y. J. AND WUT, Q. P. .2014. Detection and prevalence of pathogenic Y. enterocolitica in refrigerated and frozen dairy products by duplex PCR and dot hybridization targeting the virf and ail genes. J. Dairy Sci., 97: 6785- 6791.
YUCEL, M. N. AND ULUSOY, H. .2006. A Turkey Survey of hygienic indicator bacteria and Y. enterocolitica in raw milk and cheese sample. Food contr., 17: 383- 388.
| ||
|
Statistics Article View: 878 PDF Download: 726 |
||
