Spectrophotometric Determination of Sulfadoxine Drug use Cloud point and Flow injection Methods in Pharmaceutical Formulations | ||
| Egyptian Journal of Chemistry | ||
| Article 20, Volume 64, Issue 6, June 2021, Pages 2913-2924 PDF (891.25 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2021.55297.3164 | ||
| Authors | ||
| Nisreen Kais Abood; Mohammed Jasim M Hassana | ||
| Department of Chemistry, College of Science, Mustansiriyah University, Baghdad | ||
| Abstract | ||
| New simple sensitive spectrophotometric methods are developed for the estimation of Sulfadoxine (SFD) in pure and pharmaceutical formulations. The first method includes a conversion primary amine to azo-dye by reacting sulfadoxine with sodium nitrite and hydrochloric acid followed by coupling with4-methoxyphenol in alkaline medium to obtain a stable reddish-orange colored dye at λmax495nm. Concentration ranges 0.25-60 μg / mL, obeyed Beer's law, correlation coefficient was 0.9996, molar absorptivity was 0.589×104L.mol-1.cm-1 and the detection limit was 0.157μg/mL. The second method was cloud point extraction (CPE) for estimating trace amount in an aqueous solution that produced from diazotization and measuring with a UV-visible spectrophotometer as are reddish-purple colored product at λmax500 nm. The concentration range obeyed the Beer's law was 0.25-6μg / mL, correlation coefficient was 0.9998, molar absorptivity was 0.877×105L.mol-1.cm-1, detection limit was 0.023μg/mL, pre-concentration factor was 25 and Distribution coefficient(D) was 320.88. The last method was flow injection analysis it’s simple for estimation the sulfadoxine. The concentration range was1-150μg / mL, obeyed Beer's law,the correlation coefficient was 0.9997, molar absorptivity was 0.273×104L.mol-1.cm-1 and the detection limit was 0.375μg/mL. The offered methods were successful, useful for estimating sulfadoxine in traditional medications | ||
| Keywords | ||
| Sulfadoxine; diazotization; cloud point extraction; 4-methoxyphenol; Flow Injection Analysis | ||
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