Mohamed, F., H, O., Deif, H., Ragab, E., Eljakee, J. (2020). Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection. test, 66(1), 98-114. doi: 10.21608/vmjg.2020.157909
Fatma Elzahraa Mohamed; Omnia H; Heba Deif; Eman Ragab; Jakeen Eljakee. "Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection". test, 66, 1, 2020, 98-114. doi: 10.21608/vmjg.2020.157909
Mohamed, F., H, O., Deif, H., Ragab, E., Eljakee, J. (2020). 'Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection', test, 66(1), pp. 98-114. doi: 10.21608/vmjg.2020.157909
Mohamed, F., H, O., Deif, H., Ragab, E., Eljakee, J. Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection. test, 2020; 66(1): 98-114. doi: 10.21608/vmjg.2020.157909

Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection

Veterinary Medical Journal (Giza)
Article 9, Volume 66, Issue 1, 2020, Pages 98-114    PDF (474.54 K)
Document Type: Original Article
DOI: 10.21608/vmjg.2020.157909
Authors
Fatma Elzahraa Mohamed1; Omnia H2; Heba Deif1; Eman Ragab1; Jakeen Eljakee* 1
1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza.
2Animal Health Research institute, Giza, Egypt.
Abstract
In this work 68 S. aureus isolated frommastitic cows (n=48), camel milk (n=5) and human samples (urine n = 4, abscesses aspiration n = 3, sputum n = 2, nasal swabs n = 2, vaginal swabs n = 1 and wound swabs n = 3) were investigated using phenotypic and molecular identification to detect the occurrence of enterotoxins. Eighteen out of 48 S. aureus isolated from mastitic cows and 5 out of 15 S. aureus isolated from human samples were enterotoxin producers. All toxigenic S. aureus isolated from clinical mastitic cows (13) were toxin type D producer while seb and sed were detected from subclinical mastitic cows. Among human isolates, 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C and D and one isolate produced type C toxin. No toxin was detected from camel isolates. All toxigenic S. aureus isolated from cows (13) were toxin type D producer. Among human isolates 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C & D and one isolate produced type C toxin.  Five S. aureuscrude enterotoxin type D were investigated by protein SDS-PAGE. It was clear that all isolates had from 9 to 7 bands.  All the isolates had a band at 148-159 kDa, 35-42 kDa, 28-30 kDa, 23-26 kDa, 20-22 kDa and 18-19 kDa. 4 isolates had a band at 48-55 kDa, 3 isolates had a band at 39-42 kDa, 3 isolates had a band at 31-34 kDa and one isolate had a band at 17 kDa.
Keywords
Enterotoxin; Multiplex PCR; Mastitic milk; S. aureus; SDS
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