hassan, Y., samra, M., Helal, A., Bahnasy, A., shahin, A. (2023). Role of Some Estrogen Receptor and Her2 Pathway related Genes (PIK3CA, GSK3 β) in Predicting Response to Tamoxifen in Breast Cancer Patients. test, 29(1), 210-222. doi: 10.21608/zumj.2021.62896.2142
yasser arafat hassan; mohamed abd el-motti samra; Amany Mohamed Helal; Abeer Ahmed Bahnasy; Amgad Ahmed shahin. "Role of Some Estrogen Receptor and Her2 Pathway related Genes (PIK3CA, GSK3 β) in Predicting Response to Tamoxifen in Breast Cancer Patients". test, 29, 1, 2023, 210-222. doi: 10.21608/zumj.2021.62896.2142
hassan, Y., samra, M., Helal, A., Bahnasy, A., shahin, A. (2023). 'Role of Some Estrogen Receptor and Her2 Pathway related Genes (PIK3CA, GSK3 β) in Predicting Response to Tamoxifen in Breast Cancer Patients', test, 29(1), pp. 210-222. doi: 10.21608/zumj.2021.62896.2142
hassan, Y., samra, M., Helal, A., Bahnasy, A., shahin, A. Role of Some Estrogen Receptor and Her2 Pathway related Genes (PIK3CA, GSK3 β) in Predicting Response to Tamoxifen in Breast Cancer Patients. test, 2023; 29(1): 210-222. doi: 10.21608/zumj.2021.62896.2142

Role of Some Estrogen Receptor and Her2 Pathway related Genes (PIK3CA, GSK3 β) in Predicting Response to Tamoxifen in Breast Cancer Patients

Zagazig University Medical Journal
Article 26, Volume 29, Issue 1, January 2023, Pages 210-222    PDF (1.04 M)
Document Type: Original Article
DOI: 10.21608/zumj.2021.62896.2142
Authors
yasser arafat hassan* 1; mohamed abd el-motti samra2; Amany Mohamed Helal2; Abeer Ahmed Bahnasy3; Amgad Ahmed shahin2
1medical oncology-faculty of medicine - zagazig university-senbellawen -dakahlia
2Medical Oncology Department, National Cancer Institute, Cairo University, Cairo 11976, Egypt
3Medical Biochemistry and Molecular Biology, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo 11976, Egypt
Abstract
Background: PIK3CA and GSK-3β have important role(s) in the resistance of estrogen receptor positive (+ER) breast cancer (BC) patients to hormonal therapy. The aim of the current study was to assess the role of PIK3CA and GSK-3β in predicting response to Tamoxifen in BC patients.
Methods: PIK3CA and GSK-3β expression levels were assessed in formalin fixed paraffin embedded tissue (FFPE) sections of 58 hormonal positive BC females, using quantitative real time PCR (RT-qPCR). The data were correlated to clinico-pathological features of the patients, response to Tamoxifen and survival outcome.
Results: The median PIK3CA expression increased significantly in non-responders to tamoxifen compared to responders [2.94 (IQR=7.5) and 0.72 (IQR=0.9); respectively, P= 0.017]. However, there was no significant difference in GSK-3β expression between responders and non-responders BC patients [1.5 (IQR=3.8) and 1.2 (IQR=2.8); respectively, P= 0.27]. PIK3CA expression associated with low ER expression, presence of distant metastasis and shorter disease free survival (DFS) rate (P=0.032, 0.026 and 0.043; respectively). GSK-3β expression increased significantly in negative LN metastasis and low-expression of progesterone receptors (P=0.01 and 0.006; respectively). There was significant correlation between the expression levels of GSK-3B and PIK3CA in BC patients (r=0.88, P
Keywords
ESTROGEN; TAMOXIFEN -BREAST CANCER- PI3Kca; GsK3b
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