DEVELOPMENT OF MEDIUM FOR DIFFERENTIATION BETWEEN YERSINIA ENTEROcOLITICA AND OTHER BACTERIA | ||
| Delta Journal of Science | ||
| Article 2, Volume 28, Issue 1, July 2004, Pages 1-8 PDF (1.33 M) | ||
| Document Type: Research and Reference | ||
| DOI: 10.21608/djs.2004.158527 | ||
| Authors | ||
| S.G. ALI; M. M. AFIFI; U. M. ABDUL- RAOUF | ||
| BOTANY AND MICROBIOLOGY DEPARTMENT, FACULTY OF SCIENCE, AL-AZHAR UNIVERSITY, ASSIUT BRANCH, 71524, ASSIUT, EGYPT. | ||
| Abstract | ||
| A new dye containing medium, (Yersinia-2, 3, 5-tripheny tetrazolium chloride-roso bengal Agar, YTRA) was developed for differentiation between Yersinia enterocolitica and other bacteria ThoYTRAmedium was compared with cefs ulo din irgas an novobiocin (cIN) medium, which is tho most popular mo dium to differentiate and enumerat Yorsinia enterocolitica. Soveral combinations of twenty threo dyes woro ovaluated to develop the new medium. 2, 3, 5 Triphenyl tetrazolium chloride (0.05 g/liter) and rose bengal (0. 05 g/iter) on Brafn Hoart InfuSion Agar were found as thobest combin ation for differentiation between Yersinia enterocolitica and other bacteria. On the slightly red YTRA medium, Yersinia enterocolitica produced metallic sheen colonies, whereas other growing bacteria produced red color colonies. The recovery rates of Yersinia enterocolitica from inoculated ground beef, and milk were significantly ditferent between Cefsulodin-irgasan- novobiocin (CIN) and Yersinia- 2, 3, 5-triphenyl tetrazolium chloride-rose bengal Agar (YTRA) media. The recovery rates of heat injured Yersinia enterocolitis ere also highly Countable on YTRA medium. | ||
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