In vivo and in vitro antitumor effects of Helix desertorum hemolymph by inducing cell cycle arrest and apoptosis | ||
| Egyptian Journal of Cancer and Biomedical Research | ||
| Article 15, Volume 5, Issue 1, March 2021, Pages 155-164 PDF (3.58 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jcbr.2021.54156.1105 | ||
| Authors | ||
| Mai Ziada* ; Mohamed Mona; Mohamed Basyony; Mohamed Salem | ||
| Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt | ||
| Abstract | ||
| Background: Natural products, those extracted from invertebrate animals have medical importance as therapeutic agents especially for cancer treatment. Aim of the Work: The current study was conducted to evaluate the antitumor effect of the hemolymph of the desert snail, Helix desertorum (HD-H) on tumor Ehrlich ascetic carcinoma cells (EAC) cell line in vitro and in vivo. Materials and Methods: Helix desertorum (HD-H) was collected from their suitable habitats, identified, and then their hemolymph was collected, to evaluate their antitumor effect. 40 female albino mice were divided into five groups (n=8) as the following: group 1 (Gp1) control mice were inoculated intraperitoneal (i.p) with 1x106 EAC cells/mouseat day 0, Gp2 (EAC/Cis): Mice were injected with EAC-cells as in Gp1 and at day 2 were injected with cisplatin (40 mg/Kg), Gp3 was inoculated with EAC and then treated with splenocytes activated with HD-H, Gp4was inoculated with EAC and then treated with splenocytes activated with IL-2/Con A, and Gp5 was inoculated with EAC and then treated with splenocytes without activation. All groups were sacrificed to evaluate the tumor profile, hematological and biochemical. Results: The results showed that treatment with HD-H led to decrease tumor volume, their cell counts, increase the percentage of the apoptotic cells and arresting the cancer cell cycle. Moreover, treatment with HD-H improved the hematological and biochemical parameters on tumor-bearing mice. Conclusion: In conclusion, HD-H has potential in vitro and in vivo antitumor effects against EAC-cells. Further study is recommended to evaluate the potential efficacy of HD-H as a potential anticancer | ||
| Keywords | ||
| Anticancer; Apoptosis; Cell cycle; Helix desertorum (HD-H); Hemolymph | ||
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